In biotechnology, it is important to optimize the growth conditions of the organism. This is especially true in plant biotechnology, as plants grow slowly and produce a small amount of metabolites. Many studies have focused on the influence of visible wavelengths on growth, development and metabolite accumulation. They use LED lights and, most often, callus cultures of various plants. Plant secondary metabolites have some advantages over secondary metabolites from other kingdoms. They have higher antioxidant activity, as they must protect the plant from oxygen radicals, which are more common due to exposure to the sun. In addition, the metabolites also have antitumor, antidiabetic, anticancer and antimicrobial properties. The field of secondary metabolites in plants is poorly researched, despite the high potential and large number of biologically active substances. Metabolic pathways are poorly understood and chemical synthesis is almost impossible. In my thesis, I will focus on the influence of different wavelengths on the accumulation of secondary metabolites in callus cultures of three different species. The assignment will cover antioxidant, antidiabetic, anti-inflammatory and antiaging secondary metabolites. I will also briefly describe how light affected biomass accumulation.
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