Introduction: In recent years, the Wim Hof breathing technique has received a lot of attention from athletes as it is believed to improve physical performance. Purpose: The purpose of this thesis was investigating the impact of Wim Hof breathing technique on aerobic performance during exercise. Methods: Study involved 14 healthy participants (13 males, one female), aged 21 to 54, who were non-smokers and engaged in sports at least four times a week but did not regularly practice the Wim Hof breathing technique. Each participant completed two exercise tests in random order, one with the use of the Wim Hof breathing technique and one without. The measurements consisted of three phases: 1) Five minutes of rest, followed by the execution of the Wim Hof breathing technique or without, 2) A progressively increasing load phase until maximum workload was achieved or until the participant stopped due to discomfort or reached a respiratory quotient of 1.13, 3) Recovery phase with pedaling at 60/min and 30 W for five minutes, followed by ten minutes of rest on the bike. Throughout, heart rate, blood pressure, ventilation, oxygen consumption, CO2 exhalation, partial CO2 pressure in exhaled air (etCO2), cadence, and wattage were measured. After the test, participants rated their perceived exertion using the Borg scale. The data were statistically analyzed, and differences were considered significant at p䁤0.05. Results: The anaerobic threshold did not change with the use of the Wim Hof breathing technique compared to the control test, and VO2max did not differ between the two tests. There was a significant difference in CO2 kinetics during exercise as the values of respiratory quotient, etCO2, and VeqCO2 changed significantly at the anaerobic threshold. Participants perceived the test as less strenuous with the use of the Wim Hof breathing technique (15.21±1.58) compared to the control test (16.29±1.20), p=0.015. Discussion and Conclusion: The use of the Wim Hof breathing technique did not contribute to aerobic endurance or shift the anaerobic threshold. Future research should investigate the effects of the technique on blood lactate concentration.
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