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Small cellular particles from European spruce needle homogenate
ID
Jeran, Marko
(
Author
),
ID
Romolo, Anna
(
Author
),
ID
Spasovski, Vesna
(
Author
),
ID
Hočevar, Matej
(
Author
),
ID
Novak, Urban
(
Author
),
ID
Štukelj, Roman
(
Author
),
ID
Šuštar, Vid
(
Author
),
ID
Kisovec, Matic
(
Author
),
ID
Bedina Zavec, Apolonija
(
Author
),
ID
Kogej, Ksenija
(
Author
),
ID
Iglič, Aleš
(
Author
),
ID
Trebše, Polonca
(
Author
),
ID
Kralj-Iglič, Veronika
(
Author
)
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https://www.mdpi.com/1422-0067/24/5/4349
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Abstract
Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.
Language:
English
Keywords:
Picea abies
,
spruce needles
,
extracellular particles
,
extracellular vesicles
,
small cellular particles
,
interferometric light microscopy
,
exosomes
,
cell-to-cell communication
,
drug delivery
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
ZF - Faculty of Health Sciences
FE - Faculty of Electrical Engineering
FKKT - Faculty of Chemistry and Chemical Technology
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Publication date:
22.02.2023
Year:
2023
Number of pages:
16 str.
Numbering:
Vol. 24, no. 5, art. 4349
PID:
20.500.12556/RUL-144465
UDC:
577:615.3
ISSN on article:
1422-0067
DOI:
10.3390/ijms24054349
COBISS.SI-ID:
142622467
Publication date in RUL:
23.02.2023
Views:
625
Downloads:
76
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Record is a part of a journal
Title:
International journal of molecular sciences
Shortened title:
Int. j. mol. sci.
Publisher:
MDPI
ISSN:
1422-0067
COBISS.SI-ID:
2779162
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
22.02.2023
Secondary language
Language:
Undetermined
Keywords:
Picea abies
,
smrekove iglice
,
zunajcelični delci
,
zunajcelični vezikli
,
majhni celični delci
,
interferometrična svetlobna mikroskopija
,
eksosomi
,
komunikacija med celicami
,
dostava zdravil
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0201-2022
Name:
Fizikalna kemija
Funder:
ARRS - Slovenian Research Agency
Project number:
P2-0132-2019
Name:
Fizika in kemija površin kovinskih materialov
Funder:
ARRS - Slovenian Research Agency
Project number:
P2-0232-2022
Name:
Analiza biomedicinskih slik in signalov
Funder:
ARRS - Slovenian Research Agency
Project number:
P3-0388-2022
Name:
Mehanizmi varovanja zdravja
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0391-2022
Name:
Molekulske interakcije
Funder:
ARRS - Slovenian Research Agency
Project number:
J3-3066-2021
Name:
Optimizacija s trombociti in zunajceličnimi vezikli bogate avtologne krvne plazme za zdravljenje pooperativnih ran v otorinolaringologiji
Funder:
ARRS - Slovenian Research Agency
Project number:
L3-2621-2020
Name:
Mehanizmi omajanja endoproteze kolka
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