Acute cartilage injury induced by trans-articular sutures
ID Ciglič, Matic (Author), ID Marš, Tomaž (Author), ID Maružin, Mitja (Author), ID Alibegović, Armin (Author), ID Vesel, Miha (Author), ID Drobnič, Matej (Author)

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Objective. To determine the extent of acute cartilage injury by using trans-articular sutures. Methods. Five different absorbable sutures, monofilament polydioxanone (PDS) and braided polyglactin (Vicryl), were compared on viable human osteochondral explants. An atraumatic needle with 30 cm of thread was advanced through the cartilage with the final thread left in the tissue. A representative 300 μm transversal slice from the cartilage midportion was stained with Live/ Dead probes, scanned under the confocal laser microscope, and analyzed for the diameters of (a) central “Black zone” without any cells, representing in situ thread thickness and (b) “Green zone”, including the closest Live cells, representing the maximum injury to the tissue. The exact diameters of suture needles and threads were separately measured under an optical microscope. Results. The diameters of the Black (from 144 to 219 µm) and the Green zones (from 282 to 487 µm) varied between the different sutures (P < 0.001). The Green/Black zone ratio remained relatively constant (from 1.9 to 2.2; P = 0.767). A positive correlation between thread diameters and PDS suturing material, toward the Black and Green zone, was established, but needle diameters did not reveal any influence on the zones. Conclusions. The width of acute cartilage injury induced by the trans-articular sutures is about twice the thread thickness inside of the tissue. Less compressible monofilament PDS induced wider tissue injury in comparison to a softer braided Vicryl. Needle diameter did not correlate to the extent of acute cartilage injury.

Keywords:articular cartilage, suture, polydioxanone, polyglactin, live/dead staining
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Publication status in journal:Published
Article version:Publisher's version of article
Number of pages:Str. 318S-325S
Numbering:Vol. 13, suppl. 1
PID:20.500.12556/RUL-144117 This link opens in a new window
ISSN on article:1947-6043
DOI:10.1177/19476035211029704 This link opens in a new window
COBISS.SI-ID:113106179 This link opens in a new window
Publication date in RUL:01.02.2023
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License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

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Keywords:ortopedija, hrustanec, šivanje, patologija


Funder:Drugi - Drug financer ali več financerjev
Funding programme:University of Ljubljana, Faculty of Medicine, Institute for Pathological-Physiology, Post-doc research grant

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