Acute cartilage injury induced by trans-articular sutures

Ciglič, Matic (author), Marš, Tomaž (author), Maružin, Mitja (author), Alibegović, Armin (author), Vesel, Miha (author), Drobnič, Matej (author)

Objective. To determine the extent of acute cartilage injury by using trans-articular sutures. Methods. Five different absorbable sutures, monofilament polydioxanone (PDS) and braided polyglactin (Vicryl), were compared on viable human osteochondral explants. An atraumatic needle with 30 cm of thread was advanced through the cartilage with the final thread left in the tissue. A representative 300 μm transversal slice from the cartilage midportion was stained with Live/ Dead probes, scanned under the confocal laser microscope, and analyzed for the diameters of (a) central “Black zone” without any cells, representing in situ thread thickness and (b) “Green zone”, including the closest Live cells, representing the maximum injury to the tissue. The exact diameters of suture needles and threads were separately measured under an optical microscope. Results. The diameters of the Black (from 144 to 219 µm) and the Green zones (from 282 to 487 µm) varied between the different sutures (P < 0.001). The Green/Black zone ratio remained relatively constant (from 1.9 to 2.2; P = 0.767). A positive correlation between thread diameters and PDS suturing material, toward the Black and Green zone, was established, but needle diameters did not reveal any influence on the zones. Conclusions. The width of acute cartilage injury induced by the trans-articular sutures is about twice the thread thickness inside of the tissue. Less compressible monofilament PDS induced wider tissue injury in comparison to a softer braided Vicryl. Needle diameter did not correlate to the extent of acute cartilage injury.

• Language: English
• Keywords: articular cartilage, suture, polydioxanone, polyglactin, live/dead staining
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: Str. 318S-325S
• Numbering: Vol. 13, suppl. 1
• PID: 20.500.12556/RUL-144117
• UDC: 617.3
• ISSN on article: 1947-6043
• DOI: 10.1177/19476035211029704
• COBISS.SI-ID: 113106179
• Publication date in RUL: 01.02.2023

Record is a part of a journal

• Title: Cartilage
• Publisher: SAGE
• ISSN: 1947-6043
• COBISS.SI-ID: 26764761

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: ortopedija, hrustanec, šivanje, patologija

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: University of Ljubljana, Faculty of Medicine, Institute for Pathological-Physiology, Post-doc research grant