Estimating quantitative physiological and morphological tissue parameters of murine tumor models using hyperspectral imaging and optical profilometry

Tomanič, Tadej (avtor), Rogelj, Luka (avtor), Stergar, Jošt (avtor), Markelc, Boštjan (avtor), Božič, Tim (avtor), Kranjc Brezar, Simona (avtor), Serša, Gregor (avtor), Milanič, Matija (avtor)

Understanding tumors and their microenvironment are essential for successful and accurate disease diagnosis. Tissue physiology and morphology are altered in tumors compared to healthy tissues, and there is a need to monitor tumors and their surrounding tissues, including blood vessels, non-invasively. This preliminary study utilizes a multimodal optical imaging system combining hyperspectral imaging (HSI) and threedimensional (3D) optical profilometry (OP) to capture hyperspectral images and surface shapes of subcutaneously grown murine tumor models. Hyperspectral images are corrected with 3D OP data and analyzed using the inverseadding doubling (IAD) method to extract tissue properties such as melanin volume fraction and oxygenation. Blood vessels are segmented using the B-COSFIRE algorithm from oxygenation maps. From 3D OP data, tumor volumes are calculated and compared to manual measurements using a vernier caliper. Results show that tumors can be distinguished from healthy tissue based on most extracted tissue parameters (p< 0.05). Furthermore, blood oxygenation is 50% higher within the blood vessels than in the surrounding tissue, and tumor volumes calculated using 3D OP agree within 26% with manual measurements using a vernier caliper. Results suggest that combining HSI and OP could provide relevant quantitative information about tumors and improve the disease diagnosis.

• Jezik: Angleški jezik
• Ključne besede: medical physics, hyperspectral imaging, diffuse reflectance spectroscopy, blood vessels, tumors, diffuse reflectance spectra, inverse adding-doubling, murine models, optical profilometry
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FMF - Fakulteta za matematiko in fiziko ; ZF - Zdravstvena fakulteta ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 16 str.
• Številčenje: Vol. 16, iss. 1, art. e202200181
• PID: 20.500.12556/RUL-144024
• UDK: 616-073
• ISSN pri članku: 1864-0648
• DOI: 10.1002/jbio.202200181
• COBISS.SI-ID: 117950211
• Datum objave v RUL: 26.01.2023

TOMANIČ, Tadej, ROGELJ, Luka, STERGAR, Jošt, MARKELC, Boštjan, BOŽIČ, Tim, KRANJC BREZAR, Simona, SERŠA, Gregor in MILANIČ, Matija, 2023, Estimating quantitative physiological and morphological tissue parameters of murine tumor models using hyperspectral imaging and optical profilometry. Journal of biophotonics [na spletu]. 2023. Vol. 16, no. 1,  e202200181. [Dostopano 16 april 2025]. DOI 10.1002/jbio.202200181. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=144024

Gradivo je del revije

• Naslov: Journal of biophotonics
• Skrajšan naslov: J. biophotonics
• Založnik: Wiley
• ISSN: 1864-0648
• COBISS.SI-ID: 519053593

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: medicinska fizika, hiperspektralno slikanje, difuzna refleksijska spektroskopija, ožilje, tumorji