The use of microreactors has greatly increased in recent years due to many advantages and the use of biological catalysts has also increased as more sustainable method of production. Successful immobilization is crucial for the industrial use of enzymes. In this work, crosslinked enzyme aggregates based on laccase were prepared in a microflow system. In doing so, we tried to preserve as much enzyme activity as possible and obtain particles of suitable size with high degree of uniformity. We compared different conditions for the preparation of enzyme aggregates and managed to retain up to 66.8 % of the original enzyme activity. The prepared crosslinked enzyme aggregates were then immobilized in a membrane microreactor. In the latter, we tried to carry out the biotransformation of tyrosol acetate, which was introduced into the organic phase in the presence of oxygen in the gas phase.
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