20.500.12556/RUL-142583 Priprava zamreženih encimskih agregatov na osnovi lakaze v mikropretočnem sistemu Preparation of lacasse-based cross-linked enzyme aggregates in a microflow system Uporaba mikroreaktorjev se je v zadnjih letih močno povečala zaradi številnih prednosti, povečala pa se je tudi uporaba bioloških katalizatorjev kot bolj trajnostni način proizvodnje. Za industrijsko rabo encimov pa je ključnega pomena uspešna imobilizacija. V tem delu smo v mikropretočnem sistemu pripravili zamrežene encimske agregate na osnovi lakaze. Pri tem smo poskušali ohraniti čim večjo aktivnost encima in pridobiti delce primerne velikosti z visoko stopnjo uniformnosti. Med seboj smo primerjali različne pogoje priprave encimskih agregatov ter uspeli zadržati do 66,8 % prvotne aktivnosti encima. Pripravljene zamrežene encimske agregate smo nato imobilizirali v membranskem mikroreaktorju. V slednjem smo poskusili izvesti biotransformacijo tirozol acetata, ki smo ga v mikroreaktor uvajali v organski fazi ob prisotnosti kisika v plinski fazi. The use of microreactors has greatly increased in recent years due to many advantages and the use of biological catalysts has also increased as more sustainable method of production. Successful immobilization is crucial for the industrial use of enzymes. In this work, crosslinked enzyme aggregates based on laccase were prepared in a microflow system. In doing so, we tried to preserve as much enzyme activity as possible and obtain particles of suitable size with high degree of uniformity. We compared different conditions for the preparation of enzyme aggregates and managed to retain up to 66.8 % of the original enzyme activity. The prepared crosslinked enzyme aggregates were then immobilized in a membrane microreactor. In the latter, we tried to carry out the biotransformation of tyrosol acetate, which was introduced into the organic phase in the presence of oxygen in the gas phase. mikroreaktor imobilizacija encima lakaza biotransformacija microreactor enzyme immobilization laccase biotransformation true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2022-11-14 12:53:34 2022-11-14 12:53:41 2023-02-09 11:29:04 0000-00-00 00:00:00 2022 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2023-09-08 22121 128304899 Fabjan_Larisa_-_Priprava_zamrezenih_encimskih_agregatov_na_osnovi_lakaze_v_mikropretocnem_sistem.pdf Fabjan_Larisa_-_Priprava_zamrezenih_encimskih_agregatov_na_osnovi_lakaze_v_mikropretocnem_sistem.pdf 1 DD0AEC76244540482996C1132415BB2E 74945d99d909bf22d282ce3ee8cdd5b33b146c6b401541048640bb83389d1bd5 df4fe897-6412-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=164011 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0