Priprava zamreženih encimskih agregatov na osnovi lakaze v mikropretočnem sistemu

Fabjan, Larisa (avtor), Žnidaršič Plazl, Polona (mentor)

Uporaba mikroreaktorjev se je v zadnjih letih močno povečala zaradi številnih prednosti, povečala pa se je tudi uporaba bioloških katalizatorjev kot bolj trajnostni način proizvodnje. Za industrijsko rabo encimov pa je ključnega pomena uspešna imobilizacija. V tem delu smo v mikropretočnem sistemu pripravili zamrežene encimske agregate na osnovi lakaze. Pri tem smo poskušali ohraniti čim večjo aktivnost encima in pridobiti delce primerne velikosti z visoko stopnjo uniformnosti. Med seboj smo primerjali različne pogoje priprave encimskih agregatov ter uspeli zadržati do 66,8 % prvotne aktivnosti encima. Pripravljene zamrežene encimske agregate smo nato imobilizirali v membranskem mikroreaktorju. V slednjem smo poskusili izvesti biotransformacijo tirozol acetata, ki smo ga v mikroreaktor uvajali v organski fazi ob prisotnosti kisika v plinski fazi.

• Jezik: Slovenski jezik
• Ključne besede: mikroreaktor, imobilizacija encima, lakaza, biotransformacija
• Vrsta gradiva: Diplomsko delo/naloga
• Tipologija: 2.11 - Diplomsko delo
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Leto izida: 2022
• PID: 20.500.12556/RUL-142583
• COBISS.SI-ID: 128304899
• Datum objave v RUL: 14.11.2022

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Preparation of lacasse-based cross-linked enzyme aggregates in a microflow system

Izvleček

The use of microreactors has greatly increased in recent years due to many advantages and the use of biological catalysts has also increased as more sustainable method of production. Successful immobilization is crucial for the industrial use of enzymes. In this work, crosslinked enzyme aggregates based on laccase were prepared in a microflow system. In doing so, we tried to preserve as much enzyme activity as possible and obtain particles of suitable size with high degree of uniformity. We compared different conditions for the preparation of enzyme aggregates and managed to retain up to 66.8 % of the original enzyme activity. The prepared crosslinked enzyme aggregates were then immobilized in a membrane microreactor. In the latter, we tried to carry out the biotransformation of tyrosol acetate, which was introduced into the organic phase in the presence of oxygen in the gas phase.

• Ključne besede: microreactor, enzyme immobilization, laccase, biotransformation