Black elderberry press cake as a source of bioactive ingredients using green-based extraction approaches

Mutavski, Zorana (author), Nastić, Nataša (author), Živković, Jelena (author), Šavikin, Katarina (author), Veberič, Robert (author), Medič, Aljaž (author), Pastor, Kristian (author), Jokić, Stela (author), Vidović, Senka (author)

To study the efficiency of two green-based extraction techniques for the isolation of bioactive constituents from black elderberry press cake, changes in phenolic compounds and main anthocyanin contents were analyzed. Polyphenolic content was correlated with antioxidant and antidiabetic potential by radical-scavenging activity and monitoring of α-amylase inhibition. Black elderberry press-cake extracts were obtained by ultrasound-assisted (UAE) and microwave-assisted (MAE) extractions under different extraction conditions. High-performance liquid chromatography (HPLC) analysis revealed that cyanidin-3-sambubioside and cyanidin-3-glucoside were the principal anthocyanins in all the obtained extracts, with their content being highest in MAE obtained at 80 °C over 5 min. The same extract induced two-fold higher antioxidant activity (IC50 6.89 μg/mL) and [alpha]-amylase inhibitory potential (IC50 2.18 mg/mL) in comparison to UAE extracts. The main compositional differences between extracts obtained by the same extraction technique were assigned to the extraction temperature. A principal component analysis confirmed that the antidiabetic feature is to be attributed to the rich content of anthocyanins in black elderberry press cake. Our results indicate the great potential of underutilized black elderberry press cake for the development of novel food and herbal formulations due to notable anthocyanin contents highly correlated with antidiabetic activity.

• Language: English
• Keywords: elderberry press cake, Sambucus nigra, anthocyanins, ultrasound-assisted extraction, microwave-assisted extraction, antidiabetic activity
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 15 str.
• Numbering: Vol. 11, iss. 10, art. 1465
• PID: 20.500.12556/RUL-141869
• UDC: 543
• ISSN on article: 2079-7737
• DOI: 10.3390/ biology11101465
• COBISS.SI-ID: 124868355
• Publication date in RUL: 10.10.2022

Record is a part of a journal

• Title: Biology
• Shortened title: Biology
• Publisher: MDPI
• ISSN: 2079-7737
• COBISS.SI-ID: 523004441

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: Sambucus nigra, črni bezeg, ekstrakcija, ekstrakcijske tehnike, ultrazvok, mikrovalovi, bezgova pogača, antocianini, antidiabetično delovanje