20.500.12556/RUL-141869 Black elderberry press cake as a source of bioactive ingredients using green-based extraction approaches To study the efficiency of two green-based extraction techniques for the isolation of bioactive constituents from black elderberry press cake, changes in phenolic compounds and main anthocyanin contents were analyzed. Polyphenolic content was correlated with antioxidant and antidiabetic potential by radical-scavenging activity and monitoring of α-amylase inhibition. Black elderberry press-cake extracts were obtained by ultrasound-assisted (UAE) and microwave-assisted (MAE) extractions under different extraction conditions. High-performance liquid chromatography (HPLC) analysis revealed that cyanidin-3-sambubioside and cyanidin-3-glucoside were the principal anthocyanins in all the obtained extracts, with their content being highest in MAE obtained at 80 °C over 5 min. The same extract induced two-fold higher antioxidant activity (IC50 6.89 μg/mL) and [alpha]-amylase inhibitory potential (IC50 2.18 mg/mL) in comparison to UAE extracts. The main compositional differences between extracts obtained by the same extraction technique were assigned to the extraction temperature. A principal component analysis confirmed that the antidiabetic feature is to be attributed to the rich content of anthocyanins in black elderberry press cake. Our results indicate the great potential of underutilized black elderberry press cake for the development of novel food and herbal formulations due to notable anthocyanin contents highly correlated with antidiabetic activity. elderberry press cake Sambucus nigra anthocyanins ultrasound-assisted extraction microwave-assisted extraction antidiabetic activity Sambucus nigra črni bezeg ekstrakcija ekstrakcijske tehnike ultrazvok mikrovalovi bezgova pogača antocianini antidiabetično delovanje true false true Angleški jezik Slovenski jezik Članek v reviji 2022-10-10 10:28:57 2022-10-10 10:29:03 2023-02-23 13:12:58 0000-00-00 00:00:00 2022 0 0 15 str. iss. 10, art. 1465 Vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543 2079-7737 10.3390/ biology11101465 124868355 biology-11-01465.pdf biology-11-01465.pdf 1 70F996CE3AE52D66F27CC49A2B0AFBE0 efd4a9ac3aab8f8ac02e3ba65896cfd193a4ac148a4a4a0eb41e70f2a037268c 1dd41949-4877-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=163006 https://www.mdpi.com/2079-7737/11/10/1465 0 93035b86-4875-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=163005 Biotehniška fakulteta 0 0 0