Interactions of (−)-epigallocatechin-3-gallate with model lipid membranes

Šturm, Luka (author), Prislan, Iztok (author), González-Ortega, Rodrigo (author), Mrak, Polona (author), Snoj, Tina (author), Anderluh, Gregor (author), Poklar Ulrih, Nataša (author)

(−)-Epigallocatechin-3-gallate (EGCG) is a flavonoid known for its good antioxidant potential and health benefits. It is one of the most intriguing flavonoids, especially because of its specific interactions with model lipid membranes. It was noticed that EGCG might form EGCG rich domains/rafts at certain compositions of lipid membranes. In this article, we investigate whether EGCG forms EGCG rich domains when incorporated in 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes. Our results show that EGCG decreases lipid ordering parameter in ordered membranes and increases it in the case of disordered ones. Also, incorporation of EGCG does not affect the zeta-potential and shape of the liposomes, but it can induce aggregation of liposomes. Our study also demonstrates that liposomes with incorporated EGCG are highly protected against UV-light induced oxidation.

• Language: English
• Keywords: aggregation, liposome size, liposome shape, ultraviolet radiation, DSC, ITC, lipid-order parameter
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 13 str.
• Numbering: Vol. 1864, iss. 10, art. 183999
• PID: 20.500.12556/RUL-139600
• UDC: 577.352+577.1:547.56
• ISSN on article: 0005-2736
• DOI: 10.1016/j.bbamem.2022.183999
• COBISS.SI-ID: 112544771
• Publication date in RUL: 05.09.2022

Record is a part of a journal

• Title: Biochimica et biophysica acta. Biomembranes
• Shortened title: Biochim. biophys. acta, Biomembr.
• Publisher: Elsevier
• ISSN: 0005-2736
• COBISS.SI-ID: 4077101

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: modelne membrane, fenolne spojine, epigalokatehin, epigalokatehin-3-galat, interakcije

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0121
• Name: Biokemijska in biofizikalno-kemijska karakterizacija naravnih snovi

• Funder: ARRS - Slovenian Research Agency
• Project number: J7-2593
• Name: Razvoj bioaktivnih nanostrukturiranih vlaknatih membran za podaljšanje kakovosti svežega sadja