Barvanje bakteriofagov s fluorescein izotiocianatom

Coklin, Živa (author), Podgornik, Aleš (mentor)

Bakteriofagi so virusi, ki okužijo bakterije. Fagna terapija predstavlja alternativo zdravljenju bakterijskih okužb z antibiotiki. En od načinov za uspešno detekcijo bakteriofagov in dokazovanje infekcije sesalskih celic je kovalentna vezava fluorescentnega barvila s proteini na površini bakteriofagov. Da bi ugotovili, ali vezava barvila FITC na bakteriofage vpliva na njihovo fiziološko stanje, smo pri eksperimentalnem delu primerjali fagne rastne parametre za nepobarvane in pobarvane bakteriofage. Pobarvane bakteriofage smo uporabili tudi v samostojnem eksperimentu z namenom optimizacije protokola za določanje konstante adsorpcije.

• Language: Slovenian
• Keywords: bakteriofag T4, Escherichia coli, fluorescein izotiocianat, fagni rastni parametri, konstanta adsorpcije
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2022
• PID: 20.500.12556/RUL-139463
• COBISS.SI-ID: 125124611
• Publication date in RUL: 02.09.2022

Secondary language

• Language: English
• Title: Staining bacteriophages with fluorescein isothiocianate

Abstract

Bacteriophages are viruses that infect bacterial cells. Phage therapy is an alternative to treatment of bacterial infections with antibiotics. One method for detection of phage pathway and to prove infection of mammalian cells is covalent bonding of a fluorescent pigment to proteins on the surface of bacteriophages. In experimental work phage growth parameters were determined and compared for unstained and stained bacteriophages to determine if FITC bonding has any effect on infectivity. Stained bacteriophages were used in an independent experiment to optimise the protocol for determining the adsorption rate.

• Keywords: bacteriophage T4, Escherichia coli, fluorescein isothiocyanate, phage growth parameters, adsorption rate