20.500.12556/RUL-139463 Barvanje bakteriofagov s fluorescein izotiocianatom Staining bacteriophages with fluorescein isothiocianate Bakteriofagi so virusi, ki okužijo bakterije. Fagna terapija predstavlja alternativo zdravljenju bakterijskih okužb z antibiotiki. En od načinov za uspešno detekcijo bakteriofagov in dokazovanje infekcije sesalskih celic je kovalentna vezava fluorescentnega barvila s proteini na površini bakteriofagov. Da bi ugotovili, ali vezava barvila FITC na bakteriofage vpliva na njihovo fiziološko stanje, smo pri eksperimentalnem delu primerjali fagne rastne parametre za nepobarvane in pobarvane bakteriofage. Pobarvane bakteriofage smo uporabili tudi v samostojnem eksperimentu z namenom optimizacije protokola za določanje konstante adsorpcije. Bacteriophages are viruses that infect bacterial cells. Phage therapy is an alternative to treatment of bacterial infections with antibiotics. One method for detection of phage pathway and to prove infection of mammalian cells is covalent bonding of a fluorescent pigment to proteins on the surface of bacteriophages. In experimental work phage growth parameters were determined and compared for unstained and stained bacteriophages to determine if FITC bonding has any effect on infectivity. Stained bacteriophages were used in an independent experiment to optimise the protocol for determining the adsorption rate. bakteriofag T4 Escherichia coli fluorescein izotiocianat fagni rastni parametri konstanta adsorpcije bacteriophage T4 Escherichia coli fluorescein isothiocyanate phage growth parameters adsorption rate true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2022-09-02 12:20:07 2022-09-02 12:20:10 2022-10-11 13:14:41 0000-00-00 00:00:00 2022 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 21997 125124611 Coklin_Ziva_-_Barvanje_bakteriofagov_s_fluorescein_izotiocianatom.pdf Coklin_Ziva_-_Barvanje_bakteriofagov_s_fluorescein_izotiocianatom.pdf 1 F9EF68497D2803A9C24366DA52C1B420 1f3af57cf51e16b4c826a57a9a74eb242681f8a899b36a649c145888d699a359 c881f5cc-2aa8-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=160032 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0