Revisiting the role of pulsed electric fields in overcoming the barriers to in vivo gene electrotransfer

Sachdev, Shaurya (avtor), Potočnik, Tjaša (avtor), Rems, Lea (avtor), Miklavčič, Damijan (avtor)

Gene therapies are revolutionizing medicine by providing a way to cure hitherto incurable diseases. The scientific and technological advances have enabled the first gene therapies to become clinically approved. In addition, with the ongoing COVID-19 pandemic, we are witnessing record speeds in the development and distribution of gene-based vaccines. For gene therapy to take effect, the therapeutic nucleic acids (RNA or DNA) need to overcome several barriers before they can execute their function of producing a protein or silencing a defective or overexpressing gene. This includes the barriers of the interstitium, the cell membrane, the cytoplasmic barriers and (in case of DNA) the nuclear envelope. Gene electrotransfer (GET), i.e., transfection by means of pulsed electric fields, is a non-viral technique that can overcome these barriers in a safe and effective manner. GET has reached the clinical stage of investigations where it is currently being evaluated for its therapeutic benefits across a wide variety of indications. In this review, we formalize our current understanding of GET from a biophysical perspective and critically discuss the mechanisms by which electric field can aid in overcoming the barriers. We also identify the gaps in knowledge that are hindering optimization of GET in vivo.

• Jezik: Angleški jezik
• Ključne besede: electroporation, gene medicine, gene transfection
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 26 str.
• Številčenje: Vol. 144, art. 107994
• PID: 20.500.12556/RUL-138855
• UDK: 602.621
• ISSN pri članku: 1567-5394
• DOI: 10.1016/j.bioelechem.2021.107994
• COBISS.SI-ID: 95378435
• Datum objave v RUL: 23.08.2022

Gradivo je del revije

• Naslov: Bioelectrochemistry
• Založnik: Elsevier
• ISSN: 1567-5394
• COBISS.SI-ID: 2502484

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: elektroporacija, genska terapija, genska transfekcija