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Differences in recipient ability of uropathogenic Escherichia coli strains in relation with their pathogenic potential
ID Kuznetsova, Marina V. (Avtor), ID Maslennikova, Irina L. (Avtor), ID Pospelova, Julia S. (Avtor), ID Žgur-Bertok, Darja (Avtor), ID Starčič Erjavec, Marjanca (Avtor)

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Izvleček
Conjugation is recognized as a mechanism driving dissemination of antibacterial resistances and virulence factors among bacteria. In the presented work conjugative transfer frequency into clinical uropathogenic Escherichia coli strains (UPEC) isolated from patients with symptomatic urinary tract infections was investigated. From 93 obtained UPEC strains only 29 were suitable for conjugation experiments with the plasmid pOX38, a well-known F-plasmid derivative. The study was focused on comparison of conjugation frequencies in plankton and biofilm, including comparison of conjugation frequencies in high and low biofilm biomass with their virulence potential. It was shown that the conjugation frequency depended on the biofilm biomass and was significantly higher in thin (OD580 < 0.3) than in thick biofilm (OD580 ≥ 0.3). Nonmetric multidimensional scaling analysis revealed that higher conjugation frequencies in plankton and biofilm were directly positively correlated with the sum of virulence-associated genes of the recipient strain and presence of multidrug antibiotic resistances. On the other hand, the sum of insensitivities to different bacteriocins was negatively correlated with an increase in the conjugative transfer level. Our results obtained hence indicate that the evolution of potentially more pathogenic strains via conjugation is depended on the strains’ ability to be a “good” recipient in the conjugative transfer, possibly due to the ability to form thinner biofilms.

Jezik:Angleški jezik
Ključne besede:uropathogenic Escherichia coli strains, bacterial conjugation, biofilm, virulence-associated genes, antibiotic resistance, bacteriocin insensitivities
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2022
Št. strani:8 str.
Številčenje:Vol. 97, art. 105160
PID:20.500.12556/RUL-138850 Povezava se odpre v novem oknu
UDK:579
ISSN pri članku:1567-1348
DOI:10.1016/j.meegid.2021.105160 Povezava se odpre v novem oknu
COBISS.SI-ID:90850819 Povezava se odpre v novem oknu
Datum objave v RUL:23.08.2022
Število ogledov:648
Število prenosov:118
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
Založnik:Elsevier
ISSN:1567-1348
COBISS.SI-ID:2319124 Povezava se odpre v novem oknu

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P1-0198
Naslov:Molekularno-biološke raziskave mikroorganizmov

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:BI-RU/14-15-041

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:BI-RU/16-18-047

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Russian Foundation for Basic Research
Številka projekta:19-44-590014-р_а

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