Interactome of PTH-Regulated miRNAs and their predicted target genes for Investigating the epigenetic effects of PTH (1–34) in bone metabolism

Vrščaj, Lucija Ana (author), Marc, Janja (author), Ostanek, Barbara (author)

Osteoporosis is a metabolic bone disease that mostly affects the elderly. A lot of drugs are available, mostly with an antiresorptive effect but just a few with an osteoanabolic effect, meaning they promote bone building. PTH (1-34) or teriparatide is an osteoanabolic drug, but its efficacy varies between individuals. We performed a literature review and extracted a dataset of 62 microRNAs (miRNAs) from 10 different studies; predicted miRNA target interactions (MTIs) were obtained with the help of four software tools: DIANA, miRWalk, miRDB and TargetScan. With the construction of an interactome of PTH-regulated miRNAs and their predicted target genes, we elucidated miR-146a-5p, miR-551b-5p, miR-205-3p, miR-33a-3p, miR-338-5p as miRNAs with the most interactions and miR-410-3p as the miRNA targeting bone-related pathways with the highest significance. These miRNAs could help in further understanding the mechanism of action of PTH on bone metabolism and osteoporosis. They also have the potential for novel network-based biomarkers for osteoporosis treatment efficacy and safety and as new therapeutic targets.

• Language: English
• Keywords: osteoporosis, osteoporosis treatment, microRNA, parathyroid hormone, teriparatide, network, Cytoscape, biological pathway, gene enrichment biomarker
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Publication date: 13.08.2022
• Year: 2022
• Number of pages: Str. 1-15
• Numbering: Vol. 13, iss. 8, art. 1443
• PID: 20.500.12556/RUL-138758
• UDC: 616.71-007.234:616-085
• ISSN on article: 2073-4425
• DOI: 10.3390/genes13081443
• COBISS.SI-ID: 118226947
• Publication date in RUL: 16.08.2022

Record is a part of a journal

• Title: Genes
• Shortened title: Genes
• Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
• ISSN: 2073-4425
• COBISS.SI-ID: 523100185

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 16.08.2022

Secondary language

• Language: Slovenian
• Keywords: zdravljenje osteoporoze, mikroRNA, paratiroidni hormon, teriparatid, omrežje, biomarker za obogatitev genov, biološka pot

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0298
• Name: Geni, hormonske in osebnostne spremembe pri metabolnih motnjah

• Funder: ARRS - Slovenian Research Agency
• Project number: J3-1759
• Name: Celostna karakterizacija zadetkov analiz GWAS - pot do novih terapevtskih tarč za anabolno zdravljenje osteoporoze(GWASforAna)