Introduction: Two-dimensional printing on food is the decoration of food surfaces by using printing methods. As food as a printing substrate is a very demanding one, typically, only inkjet printing is considered a suitable printing method. Inkjet printers prints edible ink directly onto different food surfaces such as cookies, cakes, and sweets. Inks contain water as a solvent and food colourants. Typical edible ink composition consists of water, ethanol, glycol and/or glycerol as solvents and an edible colourant. Purpose: The purpose of the master's thesis is to determine if the amount of individual food colourant per unit of the product exceeds the limit value specified in the technical specification of the individual food colourant. In the next step, we developed the principal for manufacturers so they will be able to manage the risks associated with the amount of food colourants on the food surface on their own. Methods: According to the scope of the research work we determined the amount of individual food colourant on the microscope glass slide by weighing the microscope glass slides before and after printing with a 2D food printer. In our research, we used physical analysis of mass measurement with an analytical scale. Results: In the worst-case scenario, if the entire surface were printed in black, we would have reached only 0.0869 g/kg or 1.2421% of the food colourant limit value. In the best-case scenario, if the entire surface were printed in light blue, we would have reached only 0.0041 g / kg or 0.0036% of the food colourant limit value. For cakes with a lower weight, the amount of food colourants per unit will be higher than for heavier cakes. The individual percentage of food colourant per unit is lower if the entire surface is printed with a combination of different food colourants. Discussion and conclusion: Eating a cake printed with a 2D printer with any of the six food colourants (purple, light purple, blue, light blue, yellow, black) used in research or with a combination of those is safe and does not pose risks to consumer health.
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