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Extracellular vesicle enriched miR-625-3p is associated with survival of malignant mesothelioma patients
ID Goričar, Katja (Author), ID Holcar, Marija (Author), ID Mavec, Nina (Author), ID Kovač, Viljem (Author), ID Lenassi, Metka (Author), ID Dolžan, Vita (Author)

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Abstract
Malignant mesothelioma (MM) is characterized by poor prognosis and short survival. Extracellular vesicles (EVs) are membrane-bound particles released from cells into various body fluids, and their molecular composition reflects the characteristics of the origin cell. Blood EVs or their miRNA cargo might serve as new minimally invasive biomarkers that would enable earlier detection of MM or treatment outcome prediction. Our aim was to evaluate miRNAs enriched in serum EVs as potential prognostic biomarkers in MM patients in a pilot longitudinal study. EVs were isolated from serum samples obtained before and after treatment using ultracentrifugation on 20% sucrose cushion. Serum EV-enriched miR-103-3p, miR-126-3p and miR-625-3p were quantified using qPCR. After treatment, expression of miR-625-3p and miR-126-3p significantly increased in MM patients with poor treatment outcome (p = 0.012 and p = 0.036, respectively). A relative increase in miR-625-3p expression after treatment for more than 3.2% was associated with shorter progression-free survival (7.5 vs. 19.4 months, HR = 3.92, 95% CI = 1.20–12.80, p = 0.024) and overall survival (12.5 vs. 49.1 months, HR = 5.45, 95% CI = 1.06–28.11, p = 0.043) of MM patients. Bioinformatic analysis showed enrichment of 33 miR-625-3p targets in eight biological pathways. Serum EV-enriched miR-625-3p could therefore serve as a prognostic biomarker in MM and could contribute to a more personalized treatment.

Language:English
Keywords:mesothelioma, extracellular vesicles, miR-625, prognosis
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Publication status:Published
Publication version:Version of Record
Year:2021
Number of pages:18 str.
Numbering:Vol. 11, iss. 10, art. 1014
PID:20.500.12556/RUL-136266 This link opens in a new window
UDC:616-006
ISSN on article:2075-4426
DOI:10.3390/jpm11101014 This link opens in a new window
COBISS.SI-ID:80910851 This link opens in a new window
Publication date in RUL:21.04.2022
Views:556
Downloads:91
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Record is a part of a journal

Title:Journal of personalized medicine
Shortened title:J. pers. med.
Publisher:MDPI
ISSN:2075-4426
COBISS.SI-ID:31207641 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:09.10.2021

Secondary language

Language:Slovenian
Keywords:mezoteliom, zunajcelični vezikli, miR-625

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P1-0170
Name:Molekulski mehanizmi uravnavanja celičnih procesov v povezavi z nekaterimi boleznimi pri človeku

Funder:ARRS - Slovenian Research Agency
Project number:L3-8203
Name:Serumski, genetski in epigenetski označevalci tveganja za nastanek, napredovanja in odgovora na zdravljenje bolezni, povezanih z izpostavljenostjo azbestu

Funder:ARRS - Slovenian Research Agency
Project number:L3-2622
Name:Biološki označevalci nastanka, napredovanja in odgovora na zdravljenje bolezni, povezanih z izpostavljenostjo azbestu

Funder:ARRS - Slovenian Research Agency
Project number:J3-9255
Name:Ovrednotenje Nef-vsebujočih zunajceličnih veziklov v plazmi kot biooznačevalca aktivnega rezervoarja virusa HIV-1 pri aviremičnih posameznikih

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