20.500.12556/RUL-136266 Extracellular vesicle enriched miR-625-3p is associated with survival of malignant mesothelioma patients Malignant mesothelioma (MM) is characterized by poor prognosis and short survival. Extracellular vesicles (EVs) are membrane-bound particles released from cells into various body fluids, and their molecular composition reflects the characteristics of the origin cell. Blood EVs or their miRNA cargo might serve as new minimally invasive biomarkers that would enable earlier detection of MM or treatment outcome prediction. Our aim was to evaluate miRNAs enriched in serum EVs as potential prognostic biomarkers in MM patients in a pilot longitudinal study. EVs were isolated from serum samples obtained before and after treatment using ultracentrifugation on 20% sucrose cushion. Serum EV-enriched miR-103-3p, miR-126-3p and miR-625-3p were quantified using qPCR. After treatment, expression of miR-625-3p and miR-126-3p significantly increased in MM patients with poor treatment outcome (p = 0.012 and p = 0.036, respectively). A relative increase in miR-625-3p expression after treatment for more than 3.2% was associated with shorter progression-free survival (7.5 vs. 19.4 months, HR = 3.92, 95% CI = 1.20–12.80, p = 0.024) and overall survival (12.5 vs. 49.1 months, HR = 5.45, 95% CI = 1.06–28.11, p = 0.043) of MM patients. Bioinformatic analysis showed enrichment of 33 miR-625-3p targets in eight biological pathways. Serum EV-enriched miR-625-3p could therefore serve as a prognostic biomarker in MM and could contribute to a more personalized treatment. mesothelioma extracellular vesicles miR-625 prognosis mezoteliom zunajcelični vezikli miR-625 true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-21 11:04:06 2022-04-21 11:04:08 2022-09-15 04:13:40 0000-00-00 00:00:00 2021 0 0 18 str. iss. 10, art. 1014 Vol. 11 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2075-4426 10.3390/jpm11101014 80910851 jpm-11-01014-v2.pdf jpm-11-01014-v2.pdf 1 1750DFA9F081ECF3381C067F93C15640 944ff362c5146aa5e0c4775b8934bcfe01a1b57f7709516fb603b30f1450cd11 75fd6fdf-c168-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155814 https://www.mdpi.com/2075-4426/11/10/1014 1 ef550569-c151-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155810 Medicinska fakulteta 0 0 0