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Analysis of biogenic amines using immunoassays, HPLC, and a newly developed IC-MS/MS technique in fish products—a comparative study
ID
Kočar, Drago
(
Author
),
ID
Köse, Sevim
(
Author
),
ID
Koral, Serkan
(
Author
),
ID
Tufan, Bekir
(
Author
),
ID
Ščavničar, Andrej
(
Author
),
ID
Pompe, Matevž
(
Author
)
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https://www.mdpi.com/1420-3049/26/20/6156
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Abstract
In this study, comparative analyses were carried out with ion chromatography mass spectrometry (IC-MS/MS) which has no derivatization step, high-performance liquid chromatography (HPLC) technique, as well as two quantitative and two semi-quantitative immunoassays. The results demonstrated that HPLC and quantitative immunoassay methods were well-correlated with IC-MS/MS in determining histamine in various types of fish products. The best correlation was observed with the HistaSure ELISA Fast Track kit (R$^2$ = 0.9903). More than half of the values (68%) obtained by two methods were also statistically similar. The results of semi-quantitative test kits also supported histamine values estimated by quantitative methods, with some exceptions. The best results were found for HistaSure Lateral Flow in supporting the quantitative techniques. Therefore, these methods are found suitable for monitoring histamine in fish products in terms of food safety. Good correlations were also observed HPLC and IC-MS/MS in determining cadaverine, putrescine, and tyramine with the highest value observed for tyramine as R$^2$ = 0.9785. However, no correlation was observed for other biogenic amines, and the majority of the results were significantly different from each other for these amines (p < 0.05). The differences may be caused by the drawbacks reported previously for HPLC. However, further studies are required to confirm the possible effects. This study provides a comparative evaluation of several methods in terms of their suitability in determining biogenic amines in fish products for both monitoring and regulatory purposes.
Language:
English
Keywords:
biogenic amines
,
IC-MS/MS
,
HPLC
,
immunoassays
,
method comparison
,
fish products
,
histamine
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FKKT - Faculty of Chemistry and Chemical Technology
Publication status:
Published
Publication version:
Version of Record
Year:
2021
Number of pages:
18 str.
Numbering:
Vol. 26, iss. 20, art. 6156
PID:
20.500.12556/RUL-136262
UDC:
543.544:547.233
ISSN on article:
1420-3049
DOI:
10.3390/molecules26206156
COBISS.SI-ID:
81098499
Publication date in RUL:
21.04.2022
Views:
950
Downloads:
81
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Record is a part of a journal
Title:
Molecules
Shortened title:
Molecules
Publisher:
MDPI
ISSN:
1420-3049
COBISS.SI-ID:
18462981
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
12.10.2021
Secondary language
Language:
Slovenian
Keywords:
biogeni amini
,
IC-MS/MS
,
HPLC
,
imunotest
,
primerjava metod
,
ribji produkti
,
histamin
Projects
Funder:
TUBITAK - Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
Funding programme:
Uluslararası
Project number:
107O327
Name:
Geleneksel Balık Ürünlerinde Biyojenik Amin ve Nitrosaminlerin Kontrol ve İzleme Sistemleri
Funder:
ARRS - Slovenian Research Agency
Project number:
BI-TUR 2008–2010-4
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0153
Name:
Raziskave in razvoj analiznih metod in postopkov
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