Analysis of biogenic amines using immunoassays, HPLC, and a newly developed IC-MS/MS technique in fish products—a comparative study

Kočar, Drago (author), Köse, Sevim (author), Koral, Serkan (author), Tufan, Bekir (author), Ščavničar, Andrej (author), Pompe, Matevž (author)

In this study, comparative analyses were carried out with ion chromatography mass spectrometry (IC-MS/MS) which has no derivatization step, high-performance liquid chromatography (HPLC) technique, as well as two quantitative and two semi-quantitative immunoassays. The results demonstrated that HPLC and quantitative immunoassay methods were well-correlated with IC-MS/MS in determining histamine in various types of fish products. The best correlation was observed with the HistaSure ELISA Fast Track kit (R$^2$ = 0.9903). More than half of the values (68%) obtained by two methods were also statistically similar. The results of semi-quantitative test kits also supported histamine values estimated by quantitative methods, with some exceptions. The best results were found for HistaSure Lateral Flow in supporting the quantitative techniques. Therefore, these methods are found suitable for monitoring histamine in fish products in terms of food safety. Good correlations were also observed HPLC and IC-MS/MS in determining cadaverine, putrescine, and tyramine with the highest value observed for tyramine as R$^2$ = 0.9785. However, no correlation was observed for other biogenic amines, and the majority of the results were significantly different from each other for these amines (p < 0.05). The differences may be caused by the drawbacks reported previously for HPLC. However, further studies are required to confirm the possible effects. This study provides a comparative evaluation of several methods in terms of their suitability in determining biogenic amines in fish products for both monitoring and regulatory purposes.

• Language: English
• Keywords: biogenic amines, IC-MS/MS, HPLC, immunoassays, method comparison, fish products, histamine
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 18 str.
• Numbering: Vol. 26, iss. 20, art. 6156
• PID: 20.500.12556/RUL-136262
• UDC: 543.544:547.233
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules26206156
• COBISS.SI-ID: 81098499
• Publication date in RUL: 21.04.2022

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 12.10.2021

Secondary language

• Language: Slovenian
• Keywords: biogeni amini, IC-MS/MS, HPLC, imunotest, primerjava metod, ribji produkti, histamin