20.500.12556/RUL-136262 Analysis of biogenic amines using immunoassays, HPLC, and a newly developed IC-MS/MS technique in fish products—a comparative study In this study, comparative analyses were carried out with ion chromatography mass spectrometry (IC-MS/MS) which has no derivatization step, high-performance liquid chromatography (HPLC) technique, as well as two quantitative and two semi-quantitative immunoassays. The results demonstrated that HPLC and quantitative immunoassay methods were well-correlated with IC-MS/MS in determining histamine in various types of fish products. The best correlation was observed with the HistaSure ELISA Fast Track kit (R$^2$ = 0.9903). More than half of the values (68%) obtained by two methods were also statistically similar. The results of semi-quantitative test kits also supported histamine values estimated by quantitative methods, with some exceptions. The best results were found for HistaSure Lateral Flow in supporting the quantitative techniques. Therefore, these methods are found suitable for monitoring histamine in fish products in terms of food safety. Good correlations were also observed HPLC and IC-MS/MS in determining cadaverine, putrescine, and tyramine with the highest value observed for tyramine as R$^2$ = 0.9785. However, no correlation was observed for other biogenic amines, and the majority of the results were significantly different from each other for these amines (p < 0.05). The differences may be caused by the drawbacks reported previously for HPLC. However, further studies are required to confirm the possible effects. This study provides a comparative evaluation of several methods in terms of their suitability in determining biogenic amines in fish products for both monitoring and regulatory purposes. biogenic amines IC-MS/MS HPLC immunoassays method comparison fish products histamine biogeni amini IC-MS/MS HPLC imunotest primerjava metod ribji produkti histamin true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-21 09:22:35 2022-04-21 09:22:35 2022-09-15 04:13:40 0000-00-00 00:00:00 2021 0 0 18 str. iss. 20, art. 6156 Vol. 26 Oct. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544:547.233 1420-3049 10.3390/molecules26206156 81098499 molecules-26-06156-v2_(1).pdf molecules-26-06156-v2_(1).pdf 1 0E557CE149464FFD5B50118131934B73 a6cc4feb72deb9ff8258ea112276fe1709a56408bf74bd4fd3acc47b426a5abd c8b29678-c144-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155802 https://www.mdpi.com/1420-3049/26/20/6156 0 bfd8df18-c143-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155801 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0