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Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor
ID
Menegatti, Tadej
(
Author
),
ID
Žnidaršič Plazl, Polona
(
Author
)
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https://www.frontiersin.org/articles/10.3389/fbioe.2021.752064/full
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Abstract
A microbioreactor was developed in which selected amine transaminase was immobilized together with the cofactor pyridoxal phosphate (PLP) to allow efficient continuous transamination. The enzyme and cofactor were retained in a porous copolymeric hydrogel matrix formed in a two-plate microreactor with an immobilization efficiency of over 97%. After 10 days of continuous operation, 92% of the initial productivity was retained and no leaching of PLP or enzyme from the hydrogel was observed. The microbioreactor with co-immobilized cofactor showed similar performance with and without the addition of exogenous PLP, suggesting that the addition of PLP is not required during the process. The space-time yield of the microbioreactor was 19.91 g L−1 h−1, while the highest achieved biocatalyst productivity was 5.4 mg mgenzyme−1 h−1. The immobilized enzyme also showed better stability over a wider pH and temperature range than the free enzyme. Considering the time and cost efficiency of the immobilization process and the possibility of capacity expansion, such a system is of great potential for industrial application.
Language:
English
Keywords:
cofactor co-immobilization
,
hydrogel
,
microreactor
,
amine transaminase
,
continuous process
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FKKT - Faculty of Chemistry and Chemical Technology
Publication status:
Published
Publication version:
Version of Record
Publication date:
04.11.2021
Year:
2021
Number of pages:
Str. 1-10
Numbering:
Vol. 9
PID:
20.500.12556/RUL-136179
UDC:
66.098
ISSN on article:
2296-4185
DOI:
10.3389/fbioe.2021.752064
COBISS.SI-ID:
83665411
Publication date in RUL:
15.04.2022
Views:
781
Downloads:
108
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Record is a part of a journal
Title:
Frontiers in bioengineering and biotechnology
Shortened title:
Front. bioeng. biotechnol.
Publisher:
Frontiers Media
ISSN:
2296-4185
COBISS.SI-ID:
523093017
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
04.11.2021
Secondary language
Language:
Slovenian
Keywords:
koimobilizacija kofaktorja
,
hidrogel
,
mikroreaktor
,
amin transaminaza
,
kontinuirni procesi
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P2-0191
Name:
Kemijsko inženirstvo
Funder:
ARRS - Slovenian Research Agency
Project number:
J4-1775
Name:
Razvoj mikropretočnih sistemov za analizo, izbor in uporabo bakterijskih celic
Funder:
ARRS - Slovenian Research Agency
Funding programme:
Young researchers
Funder:
EC - European Commission
Funding programme:
H2020
Project number:
811040
Name:
Chair Of Micro Process Engineering and TEchnology
Acronym:
COMPETE
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