Stratified mucin-producing intraepithelial lesion (SMILE) of the uterine cervix : high-risk HPV genotype predominance and p40 immunophenotype

Strojan Fležar, Margareta (author), Nedelko, Neža (author), Poljak, Mario (author), Oštrbenk Valenčak, Anja (author), Gutnik, Helena (author)

Stratified mucin-producing intraepithelial lesion (SMILE) is a rare high-grade cervical precancerous lesion designated a variant of adenocarcinoma in situ (AIS) in the WHO classification. We aimed to determine HPV genotypes, immunohistochemical phenotype and mucin presence in SMILE. Between 2010 and 2018, SMILE was diagnosed in 34 out of 6958 (0.5%) cervical biopsies, in 23 patients. Twenty-six tissue samples from twenty-one patients were available for further analysis, including 13 with SMILE alone, 12 with SIL and/or AIS and one with HSIL, AIS and endocervical adenocarcinoma. HPV genotyping was performed using the Seegene Anyplex II HPV 28 assay. Of the 26 samples, a single HPV genotype was identified in the majority of cases (n = 22), including 12/13 SMILEs associated with SIL/AIS. All but one were high-risk HPV genotypes (23/24; 96.8%). We identified seven different HPV genotypes, the most common being HPV16 (n = 10; 43.5%), HPV18 (n = 8, 34.8%) and HPV 31 (n = 5, 21.7%). All SMILEs showed a strong positive reaction to p16, CK7, CK19 and high Ki67 expression comparable to adjacent HSIL and/or AIS if present. SMILE showed variable mucin presence and p40-positive squamous differentiation suggesting phenotypic diversity in cervical precancerous lesions infected by single HPV.

• Language: English
• Keywords: cervical precancerous lesion, intraepithelial lesions, HPV genotyping, stratified mucin-producing intraepithelial lesions, immunohistochemistry, high-risk HPV types
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 10 str.
• Numbering: Vol. 10, iss. 8, art. 2039
• PID: 20.500.12556/RUL-135981
• UDC: 616
• ISSN on article: 2073-4409
• DOI: 10.3390/cells10082039
• COBISS.SI-ID: 72916483
• Publication date in RUL: 05.04.2022

STROJAN FLEŽAR, Margareta, NEDELKO, Neža, POLJAK, Mario, OŠTRBENK VALENČAK, Anja and GUTNIK, Helena, 2021, Stratified mucin-producing intraepithelial lesion (SMILE) of the uterine cervix : high-risk HPV genotype predominance and p40 immunophenotype. Cells [online]. 2021. Vol. 10, no. 8,  2039. [Accessed 26 April 2025]. DOI 10.3390/cells10082039. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=135981

Record is a part of a journal

• Title: Cells
• Shortened title: Cells
• Publisher: MDPI
• ISSN: 2073-4409
• COBISS.SI-ID: 519958809

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 10.08.2021

Secondary language

• Language: Slovenian
• Keywords: predrakava lezija materničnega vratu, intraepitelijske lezije, genotipizacija HPV