Stratified mucin-producing intraepithelial lesion (SMILE) of the uterine cervix : high-risk HPV genotype predominance and p40 immunophenotype

Strojan Fležar, Margareta (avtor), Nedelko, Neža (avtor), Poljak, Mario (avtor), Oštrbenk Valenčak, Anja (avtor), Gutnik, Helena (avtor)

Stratified mucin-producing intraepithelial lesion (SMILE) is a rare high-grade cervical precancerous lesion designated a variant of adenocarcinoma in situ (AIS) in the WHO classification. We aimed to determine HPV genotypes, immunohistochemical phenotype and mucin presence in SMILE. Between 2010 and 2018, SMILE was diagnosed in 34 out of 6958 (0.5%) cervical biopsies, in 23 patients. Twenty-six tissue samples from twenty-one patients were available for further analysis, including 13 with SMILE alone, 12 with SIL and/or AIS and one with HSIL, AIS and endocervical adenocarcinoma. HPV genotyping was performed using the Seegene Anyplex II HPV 28 assay. Of the 26 samples, a single HPV genotype was identified in the majority of cases (n = 22), including 12/13 SMILEs associated with SIL/AIS. All but one were high-risk HPV genotypes (23/24; 96.8%). We identified seven different HPV genotypes, the most common being HPV16 (n = 10; 43.5%), HPV18 (n = 8, 34.8%) and HPV 31 (n = 5, 21.7%). All SMILEs showed a strong positive reaction to p16, CK7, CK19 and high Ki67 expression comparable to adjacent HSIL and/or AIS if present. SMILE showed variable mucin presence and p40-positive squamous differentiation suggesting phenotypic diversity in cervical precancerous lesions infected by single HPV.

• Jezik: Angleški jezik
• Ključne besede: cervical precancerous lesion, intraepithelial lesions, HPV genotyping, stratified mucin-producing intraepithelial lesions, immunohistochemistry, high-risk HPV types
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 10 str.
• Številčenje: Vol. 10, iss. 8, art. 2039
• PID: 20.500.12556/RUL-135981
• UDK: 616
• ISSN pri članku: 2073-4409
• DOI: 10.3390/cells10082039
• COBISS.SI-ID: 72916483
• Datum objave v RUL: 05.04.2022

Gradivo je del revije

• Naslov: Cells
• Skrajšan naslov: Cells
• Založnik: MDPI
• ISSN: 2073-4409
• COBISS.SI-ID: 519958809

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 10.08.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: predrakava lezija materničnega vratu, intraepitelijske lezije, genotipizacija HPV