Basic methods for preparation of liposomes and studying their interactions with different compounds, with the emphasis on polyphenols

Šturm, Luka (author), Poklar Ulrih, Nataša (author)

Studying the interactions between lipid membranes and various bioactive molecules (e.g., polyphenols) is important for determining the effects they can have on the functionality of lipid bilayers. This knowledge allows us to use the chosen compounds as potential inhibitors of bacterial and cancer cells, for elimination of viruses, or simply for keeping our healthy cells in good condition. As studying those effect can be exceedingly difficult on living cells, model lipid membranes, such as liposomes, can be used instead. Liposomal bilayer systems represent the most basic platform for studying those interactions, as they are simple, quite easy to prepare and relatively stable. They are especially useful for investigating the effects of bioactive compounds on the structure and kinetics of simple lipid membranes. In this review, we have described the most basic methods available for preparation of liposomes, as well as the essential techniques for studying the effects of bioactive compounds on those liposomes. Additionally, we have provided details for an easy laboratory implementation of some of the described methods, which should prove useful especially to those relatively new on this research field.

• Language: English
• Keywords: model lipid bilayers, bioactive compounds, membrane fluidity, membrane kinetics, essential methods for liposome/vesicle preparation
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 20 str.
• Numbering: Vol. 22, iss. 12, art. 6547
• PID: 20.500.12556/RUL-135751
• UDC: 577.352:547.56
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms22126547
• COBISS.SI-ID: 67612675
• Publication date in RUL: 30.03.2022

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 18.06.2021

Secondary language

• Language: Slovenian
• Keywords: bioaktivne spojine, modelne lipidne membrane, liposomi