Basic methods for preparation of liposomes and studying their interactions with different compounds, with the emphasis on polyphenols

Šturm, Luka (avtor), Poklar Ulrih, Nataša (avtor)

Studying the interactions between lipid membranes and various bioactive molecules (e.g., polyphenols) is important for determining the effects they can have on the functionality of lipid bilayers. This knowledge allows us to use the chosen compounds as potential inhibitors of bacterial and cancer cells, for elimination of viruses, or simply for keeping our healthy cells in good condition. As studying those effect can be exceedingly difficult on living cells, model lipid membranes, such as liposomes, can be used instead. Liposomal bilayer systems represent the most basic platform for studying those interactions, as they are simple, quite easy to prepare and relatively stable. They are especially useful for investigating the effects of bioactive compounds on the structure and kinetics of simple lipid membranes. In this review, we have described the most basic methods available for preparation of liposomes, as well as the essential techniques for studying the effects of bioactive compounds on those liposomes. Additionally, we have provided details for an easy laboratory implementation of some of the described methods, which should prove useful especially to those relatively new on this research field.

• Jezik: Angleški jezik
• Ključne besede: model lipid bilayers, bioactive compounds, membrane fluidity, membrane kinetics, essential methods for liposome/vesicle preparation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 20 str.
• Številčenje: Vol. 22, iss. 12, art. 6547
• PID: 20.500.12556/RUL-135751
• UDK: 577.352:547.56
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms22126547
• COBISS.SI-ID: 67612675
• Datum objave v RUL: 30.03.2022

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 18.06.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: bioaktivne spojine, modelne lipidne membrane, liposomi