Validated stability-indicating GC-MS method for characterization of forced degradation products of trans-caffeic acid and trans-ferulic acid

Islamčević Razboršek, Maša (avtor), Ivanović, Milena (avtor), Kolar, Mitja (avtor)

When dealing with simple phenols such as caffeic acid (CA) and ferulic acid (FA), found in a variety of plants, it is very important to have control over the most important factors that accelerate their degradation reactions. This is the first report in which the stabilities of these two compounds have been systematically tested by exposure to various different factors. Forced degradation studies were performed on pure standards (trans-CA and trans-FA), dissolved in different solvents and exposed to different oxidative, photolytic and thermal stress conditions. Additionally, a rapid, sensitive, and selective stability-indicating gas chromatographic-mass spectrometric method was developed and validated for determination of trans-CA and trans-FA in the presence of their degradation products. Cis-CA and cis-FA were confirmed as the only degradation products in all the experiments performed. All the compounds were perfectly separated by gas chromatography (GC) and identified using mass spectrometry (MS), a method that additionally elucidated their structures. In general, more protic solvents, higher temperatures, UV radiation and longer storage times led to more significant degradation (isomerization) of both trans-isomers. The most progressive isomerization of both compounds (up to 43%) was observed when the polar solutions were exposed to daylight at room temperature for 1 month. The method was validated for linearity, precision as repeatability, limit of detection (LOD) and limit of quantitation (LOQ). The method was confirmed as linear over tested concentration ranges from 1−100 mg L$^{−1}$ (r$^2$s were above 0.999). The LOD and LOQ for trans-FA were 0.15 mg L$^{−1}$ and 0.50 mg L$^{−1}$, respectively. The LOD and LOQ for trans-CA were 0.23 mg L$^{−1}$ and 0.77 mg L$^{−1}$, respectively.

• Jezik: Angleški jezik
• Ključne besede: caffeic acid, ferulic acid, stability studies, GC-MS, forced degradation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 12 str.
• Številčenje: Vol. 26, iss. 9, art. 2475
• PID: 20.500.12556/RUL-135298
• UDK: 66
• ISSN pri članku: 1420-3049
• DOI: 10.3390/molecules26092475
• COBISS.SI-ID: 61605891
• Datum objave v RUL: 07.03.2022

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.05.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kofeinska kislina, prisilna degradacija, študija stabilnosti

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P2-0006
• Naslov: Fizikalno kemijski pojavi na površinskih plasteh in uporaba nanodelcev

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0153
• Naslov: Raziskave in razvoj analiznih metod in postopkov