Identification and quantification of major phenolic constituents in Juglans regia L. leaves : healthy vs. infected leaves with Xanthomonas campestris pv. juglandis using HPLC- MS/MS

Medič, Aljaž (author), Jakopič, Jerneja (author), Hudina, Metka (author), Solar, Anita (author), Veberič, Robert (author)

The present study was designed to characterise and quantify the major phenolic constituents in healthy leaves and leaves infected with Xanthomonas campestris pv. juglandis. A comparison among six different cultivars: 'Fernor', 'Fernette', 'Franquette', 'Rubina', 'Sava' and 'Krka', with the same agricultural, geographical and climatic conditions, was made. Liquid chromatography coupled with a mass spectrometer (HPLC-MS/MS) was used to identify and quantify the compounds. A total of 52 compounds were identified based on mass spectra and literature. Among them, 15 hydroxycinnamic acids, 6 flavanols, 2 flavones, 22 flavonols and 7 naphthoquinones were identified. Two flavones and three naphthoquinones were reported for the first time in J. regia leaves. In addition, two naphthoquinones, which are reported to play an active role in the process of juglone formation, were confirmed in all six cultivars. In the process of MS fragmentation, compounds were fragmented up to MS$^6$ fragments and in some cases both MS$^2$ fragments were further fragmented, providing comprehensive data. Total analysed phenolic content (TAPC) and total phenolic content (TPC) concentrations were higher in infected leaves, suggesting that phenols play a major role in plant defence. In the case of walnut bacterial blight, the contents of flavanols and total hydroxycinnamic acids were higher in infected leaves in all cultivars, suggesting that they could play a key role in a plant’s response to this economically important disease.

• Language: English
• Keywords: Juglans regia, persian walnut, leaves, Xanthomonas campestris pv. juglandis, phenolic compounds, hydrojuglone, juglone, 1, 4-naphthoquinone, naphthoquinones
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 9 str.
• Numbering: Vol. 34, iss. 3, art. 101890
• PID: 20.500.12556/RUL-134978
• UDC: 634.1/.7
• ISSN on article: 2213-686X
• DOI: 10.1016/j.jksus.2022.101890
• COBISS.SI-ID: 96667651

Note

Založnik na prvi strani članka navaja ISSN 1018-3647, ki velja za različico te revije v arabskem jeziku.

• Publication date in RUL: 16.02.2022

Record is a part of a journal

• Title: Journal of King Saud University. Elektronski vir, Science
• Publisher: Elsevier
• ISSN: 2213-686X
• COBISS.SI-ID: 19155203

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: oreh, Juglans regia, Xanthomonas campestris pv. juglandis, okuženi listi, zdravi listi, fenolne spojine, juglon, hidrojuglon, 1, 4-naftokinon

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0013
• Name: Hortikultura