20.500.12556/RUL-134978 Identification and quantification of major phenolic constituents in Juglans regia L. leaves healthy vs. infected leaves with Xanthomonas campestris pv. juglandis using HPLC- MS/MS The present study was designed to characterise and quantify the major phenolic constituents in healthy leaves and leaves infected with Xanthomonas campestris pv. juglandis. A comparison among six different cultivars: 'Fernor', 'Fernette', 'Franquette', 'Rubina', 'Sava' and 'Krka', with the same agricultural, geographical and climatic conditions, was made. Liquid chromatography coupled with a mass spectrometer (HPLC-MS/MS) was used to identify and quantify the compounds. A total of 52 compounds were identified based on mass spectra and literature. Among them, 15 hydroxycinnamic acids, 6 flavanols, 2 flavones, 22 flavonols and 7 naphthoquinones were identified. Two flavones and three naphthoquinones were reported for the first time in J. regia leaves. In addition, two naphthoquinones, which are reported to play an active role in the process of juglone formation, were confirmed in all six cultivars. In the process of MS fragmentation, compounds were fragmented up to MS$^6$ fragments and in some cases both MS$^2$ fragments were further fragmented, providing comprehensive data. Total analysed phenolic content (TAPC) and total phenolic content (TPC) concentrations were higher in infected leaves, suggesting that phenols play a major role in plant defence. In the case of walnut bacterial blight, the contents of flavanols and total hydroxycinnamic acids were higher in infected leaves in all cultivars, suggesting that they could play a key role in a plant’s response to this economically important disease. Juglans regia persian walnut leaves Xanthomonas campestris pv. juglandis phenolic compounds hydrojuglone juglone 1 4-naphthoquinone naphthoquinones oreh Juglans regia Xanthomonas campestris pv. juglandis okuženi listi zdravi listi fenolne spojine juglon hidrojuglon 1 4-naftokinon true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-16 08:53:59 2022-02-16 08:54:05 2022-09-19 09:41:22 0000-00-00 00:00:00 2022 0 0 Založnik na prvi strani članka navaja ISSN 1018-3647, ki velja za različico te revije v arabskem jeziku. 9 str. iss. 3, art. 101890 Vol. 34 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.1/.7 2213-686X 10.1016/j.jksus.2022.101890 96667651 RAZ_Medic_Aljaz_2022.pdf RAZ_Medic_Aljaz_2022.pdf 1 49C1B247D4DB2E27CBA77C28BA0AE7FF 6bb9778c5625ad9c1dc27e956d742af2cc0ea22ad3d3fe7ec02625429042de8d fb6001b7-8efd-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153775 https://www.sciencedirect.com/science/article/pii/S1018364722000714 0 5e8b7e78-8efd-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153774 Biotehniška fakulteta 0 0 0