The aim of the diploma thesis is a literature review of O-glycan profiling in biosimilars. Glycans represent one of the main elements in planning new biosimilars and vaccines using antibodies, that is why they play an important role in the pharmaceutical industry. The shortage of analytical tools for selective release of O-glycans represents a big problem in Glycan profiling. The discovery of an universal O-glycosidase or a reliable method for the release of O-attached oligosaccharides would have a lot of advantages. Currently, the most common method is the chemical release of O-glycans under alkaline conditions, which gives satisfactory results, but the method itself is not selective, as N-bound glycans are also released. The released O-glycans can be tagged, derivatized or analysed with HPLC, MS or LC-MS methods and the O-glycopeptides can be analysed with MS or LC-MS methods, but these still represent a technological challange for us. Good knowledge about glycans and their analysis are crucial for both healthcare and the pharmaceutical industry, in order to develop and ensure the quality and safety of new drugs.
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