Are metal ions that make up orthodontic alloys cytotoxic, and do they induce oxidative stress in a yeast cell model?

Kovač, Vito (author), Poljšak, Borut (author), Primožič, Jasmina (author), Jamnik, Polona (author)

Compositions of stainless steel, nickel-titanium, cobalt-chromium and β-titanium orthodontic alloys were simulated with mixtures of Fe, Ni, Cr, Co, Ti and Mo metal ions as potential oxidative stress-triggering agents. Wild-type yeast Saccharomyces cerevisiae and two mutants ∆Sod1 and ∆Ctt1 were used as model organisms to assess the cytotoxicity and oxidative stress occurrence. Metal mixtures at concentrations of 1, 10, 100 and 1000 µM were prepared out of metal chlorides and used to treat yeast cells for 24 h. Every simulated orthodontic alloy at 1000 µM was cytotoxic, and, in the case of cobalt-chromium alloy, even 100 µM was cytotoxic. Reactive oxygen species and oxidative damage were detected for stainless steel and both cobalt-chromium alloys at 1000 µM in wild-type yeast and 100 µM in the ∆Sod1 and ∆Ctt1 mutants. Simulated nickel-titanium and β-titanium alloy did not induce oxidative stress in any of the tested strains.

• Language: English
• Keywords: metal ion, orthodontic appliances, yeast, cytotoxicity, oxidative stress, lipid oxidation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences; MF - Faculty of Medicine; BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 15 str.
• Numbering: Vol. 21, iss. 21, art. 7993
• PID: 20.500.12556/RUL-134717
• UDC: 616.314-089.23:546.73/.76:549.261/.263:582.283.23
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms21217993
• COBISS.SI-ID: 34996227
• Publication date in RUL: 27.01.2022

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.11.2020

Secondary language

• Language: Slovenian
• Keywords: ortodontski pripomočki, kovinski ioni, kvasovke, citotoksičnost, oksidativni stres

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0388
• Name: Mehanizmi varovanja zdravja

• Funder: ARRS - Slovenian Research Agency
• Project number: J3-2523
• Name: Disregulacija presnove v astrogliji pri nevrodegeneraciji