Are metal ions that make up orthodontic alloys cytotoxic, and do they induce oxidative stress in a yeast cell model?

Kovač, Vito (avtor), Poljšak, Borut (avtor), Primožič, Jasmina (avtor), Jamnik, Polona (avtor)

Compositions of stainless steel, nickel-titanium, cobalt-chromium and β-titanium orthodontic alloys were simulated with mixtures of Fe, Ni, Cr, Co, Ti and Mo metal ions as potential oxidative stress-triggering agents. Wild-type yeast Saccharomyces cerevisiae and two mutants ∆Sod1 and ∆Ctt1 were used as model organisms to assess the cytotoxicity and oxidative stress occurrence. Metal mixtures at concentrations of 1, 10, 100 and 1000 µM were prepared out of metal chlorides and used to treat yeast cells for 24 h. Every simulated orthodontic alloy at 1000 µM was cytotoxic, and, in the case of cobalt-chromium alloy, even 100 µM was cytotoxic. Reactive oxygen species and oxidative damage were detected for stainless steel and both cobalt-chromium alloys at 1000 µM in wild-type yeast and 100 µM in the ∆Sod1 and ∆Ctt1 mutants. Simulated nickel-titanium and β-titanium alloy did not induce oxidative stress in any of the tested strains.

• Jezik: Angleški jezik
• Ključne besede: metal ion, orthodontic appliances, yeast, cytotoxicity, oxidative stress, lipid oxidation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta ; MF - Medicinska fakulteta ; BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 15 str.
• Številčenje: Vol. 21, iss. 21, art. 7993
• PID: 20.500.12556/RUL-134717
• UDK: 616.314-089.23:546.73/.76:549.261/.263:582.283.23
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms21217993
• COBISS.SI-ID: 34996227
• Datum objave v RUL: 27.01.2022

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.11.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ortodontski pripomočki, kovinski ioni, kvasovke, citotoksičnost, oksidativni stres

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0388
• Naslov: Mehanizmi varovanja zdravja

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-2523
• Naslov: Disregulacija presnove v astrogliji pri nevrodegeneraciji