izpis_h1_title_alt

Haloadaptative responses of Aspergillus sydowii to extreme water deprivation : morphology, compatible solutes, and oxidative stress at NaCl saturation
ID Jiménez-Gómez, Irina (Author), ID Valdés-Muñoz, Gisell (Author), ID Moreno-Perlín, Tonatiuh (Author), ID Mouriño-Pérez, Rosa R. (Author), ID Rayo Sánchez-Carbente, María del (Author), ID Folch-Mallol, Jorge Luis (Author), ID Pérez-Llano, Yordanis (Author), ID Gunde-Cimerman, Nina (Author), ID C. Sánchez, Nilda del (Author), ID Batista-García, Ramón Alberto (Author)

.pdfPDF - Presentation file, Download (2,91 MB)
MD5: 39C329DE24DEF42EB22E6C323C8048C2
URLURL - Source URL, Visit https://www.mdpi.com/2309-608X/6/4/316 This link opens in a new window

Abstract
Water activity (a$_w$) is critical for microbial growth, as it is severely restricted at a$_w$ < 0.90. Saturating NaCl concentrations (~5.0 M) induce extreme water deprivation (aw ≅ 0.75) and cellular stress responses. Halophilic fungi have cellular adaptations that enable osmotic balance and ionic/oxidative stress prevention to grow at high salinity. Here we studied the morphology, osmolyte synthesis, and oxidative stress defenses of the halophile Aspergillus sydowii EXF-12860 at 1.0 M and 5.13 M NaCl. Colony growth, pigmentation, exudate, and spore production were inhibited at NaCl-saturated media. Additionally, hyphae showed unpolarized growth, lower diameter, and increased septation, multicellularity and branching compared to optimal NaCl concentration. Trehalose, mannitol, arabitol, erythritol, and glycerol were produced in the presence of both 1.0 M and 5.13 M NaCl. Exposing A. sydowii cells to 5.13 M NaCl resulted in oxidative stress evidenced by an increase in antioxidant enzymes and lipid peroxidation biomarkers. Also, genes involved in cellular antioxidant defense systems were upregulated. This is the most comprehensive study that investigates the micromorphology and the adaptative cellular response of different non-enzymatic and enzymatic oxidative stress biomarkers in halophilic filamentous fungi.

Language:English
Keywords:Aspergillus sydowii, halophily, extremophilic fungi, saline stress, saturated NaCl solution, water deprivation, oxidative stress, morphology
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2020
Number of pages:20 str.
Numbering:Vol. 6, iss. 4, art. 316
PID:20.500.12556/RUL-134689 This link opens in a new window
UDC:577
ISSN on article:2309-608X
DOI:10.3390/jof6040316 This link opens in a new window
COBISS.SI-ID:46917379 This link opens in a new window
Publication date in RUL:26.01.2022
Views:1314
Downloads:122
Metadata:XML RDF-CHPDL DC-XML DC-RDF
:
Copy citation
Share:Bookmark and Share

Record is a part of a journal

Title:Journal of Fungi
Shortened title:J. Fungi
Publisher:MDPI
ISSN:2309-608X
COBISS.SI-ID:525300761 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.12.2020

Secondary language

Language:Slovenian
Keywords:biokemija, glive, oksidativni stres, morfologija

Projects

Funder:Other - Other funder or multiple funders
Funding programme:Mexico, Consejo Nacional de Ciencia y Tecnología, Conacyt Vocaciones Científicas
Project number:1004

Funder:Other - Other funder or multiple funders
Funding programme:Mexico, Consejo Nacional de Ciencia y Tecnología
Project number:Conacyt-SEP-CB-285816

Funder:Other - Other funder or multiple funders
Funding programme:Mexico, Consejo Nacional de Ciencia y Tecnología
Project number:Conacyt-DADC-311684

Funder:Other - Other funder or multiple funders
Funding programme:Mexico, Consejo Nacional de Ciencia y Tecnología, PhD fellowship

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Back