20.500.12556/RUL-134689 Haloadaptative responses of Aspergillus sydowii to extreme water deprivation morphology, compatible solutes, and oxidative stress at NaCl saturation Water activity (a$_w$) is critical for microbial growth, as it is severely restricted at a$_w$ < 0.90. Saturating NaCl concentrations (~5.0 M) induce extreme water deprivation (aw ≅ 0.75) and cellular stress responses. Halophilic fungi have cellular adaptations that enable osmotic balance and ionic/oxidative stress prevention to grow at high salinity. Here we studied the morphology, osmolyte synthesis, and oxidative stress defenses of the halophile Aspergillus sydowii EXF-12860 at 1.0 M and 5.13 M NaCl. Colony growth, pigmentation, exudate, and spore production were inhibited at NaCl-saturated media. Additionally, hyphae showed unpolarized growth, lower diameter, and increased septation, multicellularity and branching compared to optimal NaCl concentration. Trehalose, mannitol, arabitol, erythritol, and glycerol were produced in the presence of both 1.0 M and 5.13 M NaCl. Exposing A. sydowii cells to 5.13 M NaCl resulted in oxidative stress evidenced by an increase in antioxidant enzymes and lipid peroxidation biomarkers. Also, genes involved in cellular antioxidant defense systems were upregulated. This is the most comprehensive study that investigates the micromorphology and the adaptative cellular response of different non-enzymatic and enzymatic oxidative stress biomarkers in halophilic filamentous fungi. Aspergillus sydowii halophily extremophilic fungi saline stress saturated NaCl solution water deprivation oxidative stress morphology biokemija glive oksidativni stres morfologija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-26 12:07:01 2022-01-26 12:07:03 2022-09-13 03:56:34 0000-00-00 00:00:00 2020 0 0 20 str. iss. 4, art. 316 Vol. 6 Dec. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2309-608X 10.3390/jof6040316 46917379 RAZ_Jimenez-Gomez_Irina_2020.pdf RAZ_Jimenez-Gomez_Irina_2020.pdf 1 39C329DE24DEF42EB22E6C323C8048C2 bfeb4ab893e0abe4f063797383c16f7f3efc122130e48f883ac3112e8ff4717b e704dceb-7e97-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153260 https://www.mdpi.com/2309-608X/6/4/316 0 198d93c1-7e98-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153261 Biotehniška fakulteta 0 0 0