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Expression of extracellular matrix-related genes and their regulatory microRNAs in problematic colorectal polyps
ID
Žlajpah, Margareta
(
Author
),
ID
Boštjančič, Emanuela
(
Author
),
ID
Tepeš, Bojan
(
Author
),
ID
Zidar, Nina
(
Author
)
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https://www.mdpi.com/2072-6694/12/12/3715
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Abstract
Colorectal carcinoma usually evolves gradually, forming a spectrum of lesions, due to accumulation of genetic mutations and epigenetic alterations. Many early lesions are detected since the introduction of screening programs. The greatest challenge is to distinguish between adenomas with epithelial misplacement (AEM) and adenomas with early carcinoma (AEC), considering the diagnosis affects prognosis and treatment. We analyzed the expression of selected extracellular matrix (ECM)-related genes and proteins, and their regulatory microRNAs using RT-qPCR and immunohistochemistry in biopsies from 44 patients. Differences were observed in AEM in comparison to AEC for DCN, EPHA4, FN1, SPON2, and SPP1, reflecting inflammatory stromal reaction to traumatisation and misplacement of dysplastic glands in the submucosa in the former, and desmoplastic stromal reaction to true invasion of dysplastic glands in the submucosa in the latter. Expression of regulatory microRNAs hsa-miR-200c and hsa-miR-146a significantly negatively correlated with the expression of their regulated genes, while significant difference between AEM and AEC was observed only for hsa-miR-29c. The described expression patterns are too complex to be used in diagnostic work, but might contribute to better understanding ECM changes in colorectal carcinoma development, helping to find new markers in the future.
Language:
English
Keywords:
extracellular matrix
,
colorectal carcinoma
,
colorectal polyps
,
adenoma with epithelial misplacement
,
adenoma with early carcinoma
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2020
Number of pages:
15 str.
Numbering:
Vol. 12, iss. 12, art. 3715
PID:
20.500.12556/RUL-134672
UDC:
616-006
ISSN on article:
2072-6694
DOI:
10.3390/cancers12123715
COBISS.SI-ID:
42771715
Publication date in RUL:
25.01.2022
Views:
681
Downloads:
134
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Record is a part of a journal
Title:
Cancers
Shortened title:
Cancers
Publisher:
MDPI
ISSN:
2072-6694
COBISS.SI-ID:
517914137
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
11.12.2020
Secondary language
Language:
Slovenian
Keywords:
zunajcelični matriks
,
kolorektalni karcinom
,
kolorektalni polipi
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P3-0054
Name:
Patologija in molekularna genetika
Funder:
ARRS - Slovenian Research Agency
Project number:
J3-1754
Name:
Novi prognostični in diagnostični označevalci v razvoju raka debelega črevesa in danke
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