Expression of extracellular matrix-related genes and their regulatory microRNAs in problematic colorectal polyps

Žlajpah, Margareta (author), Boštjančič, Emanuela (author), Tepeš, Bojan (author), Zidar, Nina (author)

Colorectal carcinoma usually evolves gradually, forming a spectrum of lesions, due to accumulation of genetic mutations and epigenetic alterations. Many early lesions are detected since the introduction of screening programs. The greatest challenge is to distinguish between adenomas with epithelial misplacement (AEM) and adenomas with early carcinoma (AEC), considering the diagnosis affects prognosis and treatment. We analyzed the expression of selected extracellular matrix (ECM)-related genes and proteins, and their regulatory microRNAs using RT-qPCR and immunohistochemistry in biopsies from 44 patients. Differences were observed in AEM in comparison to AEC for DCN, EPHA4, FN1, SPON2, and SPP1, reflecting inflammatory stromal reaction to traumatisation and misplacement of dysplastic glands in the submucosa in the former, and desmoplastic stromal reaction to true invasion of dysplastic glands in the submucosa in the latter. Expression of regulatory microRNAs hsa-miR-200c and hsa-miR-146a significantly negatively correlated with the expression of their regulated genes, while significant difference between AEM and AEC was observed only for hsa-miR-29c. The described expression patterns are too complex to be used in diagnostic work, but might contribute to better understanding ECM changes in colorectal carcinoma development, helping to find new markers in the future.

• Language: English
• Keywords: extracellular matrix, colorectal carcinoma, colorectal polyps, adenoma with epithelial misplacement, adenoma with early carcinoma
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 15 str.
• Numbering: Vol. 12, iss. 12, art. 3715
• PID: 20.500.12556/RUL-134672
• UDC: 616-006
• ISSN on article: 2072-6694
• DOI: 10.3390/cancers12123715
• COBISS.SI-ID: 42771715
• Publication date in RUL: 25.01.2022

Record is a part of a journal

• Title: Cancers
• Shortened title: Cancers
• Publisher: MDPI
• ISSN: 2072-6694
• COBISS.SI-ID: 517914137

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 11.12.2020

Secondary language

• Language: Slovenian
• Keywords: zunajcelični matriks, kolorektalni karcinom, kolorektalni polipi

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0054
• Name: Patologija in molekularna genetika

• Funder: ARRS - Slovenian Research Agency
• Project number: J3-1754
• Name: Novi prognostični in diagnostični označevalci v razvoju raka debelega črevesa in danke