Simplified LC-MS method for analysis of sterols in biological samples

Skubic, Cene (author), Vovk, Irena (author), Rozman, Damjana (author), Križman, Mitja (author)

We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes in a single chromatographic run using a pentafluorophenyl (PFP) stationary phase. The method also avails on a minimized sample preparation procedure in order to obtain a relatively high sample throughput. The method was validated on 10 sterol standards that were detected in a single chromatographic LC-MS run without derivatization. Our developed method can be used in research or clinical applications for disease-related detection of accumulated cholesterol intermediates. Disorders in the late part of cholesterol synthesis lead to severe malformation in human patients. The developed method enables a simple, sensitive, and fast quantification of sterols, without the need of extended knowledge of the LC-MS technique, and represents a new analytical tool in the rising field of cholesterolomics.

• Language: English
• Keywords: cholesterol, cholesterol synthesis, sterol intermediates, LC-MS, pentafluorophenyl, PFP
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 10 str.
• Numbering: Vol. 25, iss. 18, art. 4116
• PID: 20.500.12556/RUL-134649
• UDC: 616.1
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules25184116
• COBISS.SI-ID: 28345859
• Publication date in RUL: 24.01.2022

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 09.09.2020

Secondary language

• Language: Slovenian
• Keywords: holesterol, sinteza holesterola, sterolni intermediati