Your browser does not allow JavaScript!
JavaScript is necessary for the proper functioning of this website. Please enable JavaScript or use a modern browser.
Open Science Slovenia
Open Science
DiKUL
slv
|
eng
Search
Browse
New in RUL
About RUL
In numbers
Help
Sign in
Simplified LC-MS method for analysis of sterols in biological samples
ID
Skubic, Cene
(
Author
),
ID
Vovk, Irena
(
Author
),
ID
Rozman, Damjana
(
Author
),
ID
Križman, Mitja
(
Author
)
PDF - Presentation file,
Download
(1,17 MB)
MD5: 4A845CF08ACB8B4338F3E92CD3B46EFB
URL - Source URL, Visit
https://www.mdpi.com/1420-3049/25/18/4116
Image galllery
Abstract
We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes in a single chromatographic run using a pentafluorophenyl (PFP) stationary phase. The method also avails on a minimized sample preparation procedure in order to obtain a relatively high sample throughput. The method was validated on 10 sterol standards that were detected in a single chromatographic LC-MS run without derivatization. Our developed method can be used in research or clinical applications for disease-related detection of accumulated cholesterol intermediates. Disorders in the late part of cholesterol synthesis lead to severe malformation in human patients. The developed method enables a simple, sensitive, and fast quantification of sterols, without the need of extended knowledge of the LC-MS technique, and represents a new analytical tool in the rising field of cholesterolomics.
Language:
English
Keywords:
cholesterol
,
cholesterol synthesis
,
sterol intermediates
,
LC-MS
,
pentafluorophenyl
,
PFP
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2020
Number of pages:
10 str.
Numbering:
Vol. 25, iss. 18, art. 4116
PID:
20.500.12556/RUL-134649
UDC:
616.1
ISSN on article:
1420-3049
DOI:
10.3390/molecules25184116
COBISS.SI-ID:
28345859
Publication date in RUL:
24.01.2022
Views:
717
Downloads:
125
Metadata:
Cite this work
Plain text
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Copy citation
Share:
Record is a part of a journal
Title:
Molecules
Shortened title:
Molecules
Publisher:
MDPI
ISSN:
1420-3049
COBISS.SI-ID:
18462981
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
09.09.2020
Secondary language
Language:
Slovenian
Keywords:
holesterol
,
sinteza holesterola
,
sterolni intermediati
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0390
Name:
Funkcijska genomika in biotehnologija za zdravje
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0005
Name:
Funkcionalna živila in prehranska dopolnila
Funder:
ARRS - Slovenian Research Agency
Project number:
J1-9176
Name:
HolesteROR pri presnovnih boleznih jeter
Similar documents
Similar works from RUL:
Similar works from other Slovenian collections:
Back