20.500.12556/RUL-134649 Simplified LC-MS method for analysis of sterols in biological samples We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes in a single chromatographic run using a pentafluorophenyl (PFP) stationary phase. The method also avails on a minimized sample preparation procedure in order to obtain a relatively high sample throughput. The method was validated on 10 sterol standards that were detected in a single chromatographic LC-MS run without derivatization. Our developed method can be used in research or clinical applications for disease-related detection of accumulated cholesterol intermediates. Disorders in the late part of cholesterol synthesis lead to severe malformation in human patients. The developed method enables a simple, sensitive, and fast quantification of sterols, without the need of extended knowledge of the LC-MS technique, and represents a new analytical tool in the rising field of cholesterolomics. cholesterol cholesterol synthesis sterol intermediates LC-MS pentafluorophenyl PFP holesterol sinteza holesterola sterolni intermediati true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-24 08:42:09 2022-01-24 08:42:15 2022-09-13 03:56:19 0000-00-00 00:00:00 2020 0 0 10 str. iss. 18, art. 4116 Vol. 25 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.1 1420-3049 10.3390/molecules25184116 28345859 RAZ_Skubic_Cene_2020.pdf RAZ_Skubic_Cene_2020.pdf 1 4A845CF08ACB8B4338F3E92CD3B46EFB 00ab9fcdeb3a98ac9d4958ee196361542cf2e6829d17fb9db2248bc44b19bad7 4aa8382d-7ce9-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153186 https://www.mdpi.com/1420-3049/25/18/4116 1 f3ea4155-7ce8-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153185 Medicinska fakulteta 0 0 0