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Analysis of miR-9-5p, miR-124-3p, miR-21-5p, miR-138-5p, and miR-1-3p in glioblastoma cell lines and extracellular vesicles
ID
Zottel, Alja
(
Author
),
ID
Šamec, Neja
(
Author
),
ID
Kump, Ana
(
Author
),
ID
Raspor Dall'Olio, Lucija
(
Author
),
ID
Pužar Dominkuš, Pia
(
Author
),
ID
Romih, Rok
(
Author
),
ID
Hudoklin, Samo
(
Author
),
ID
Mlakar, Jernej
(
Author
),
ID
Nikitin, Daniil
(
Author
),
ID
Sorokin, Maxim
(
Author
),
ID
Buzdin, Anton A.
(
Author
),
ID
Jovchevska, Ivana
(
Author
),
ID
Komel, Radovan
(
Author
)
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https://www.mdpi.com/1422-0067/21/22/8491
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Abstract
Glioblastoma (GBM), the most common primary brain tumor, is a complex and extremely aggressive disease. Despite recent advances in molecular biology, there is a lack of biomarkers, which would improve GBM's diagnosis, prognosis, and therapy. Here, we analyzed by qPCR the expression levels of a set of miRNAs in GBM and lower-grade glioma human tissue samples and performed a survival analysis in silico. We then determined the expression of same miRNAs and their selected target mRNAs in small extracellular vesicles (sEVs) of GBM cell lines. We showed that the expression of miR-21-5p was significantly increased in GBM tissue compared to lower-grade glioma and reference brain tissue, while miR-124-3p and miR-138-5p were overexpressed in reference brain tissue compared to GBM. We also demonstrated that miR-9-5p and miR-124-3p were overexpressed in the sEVs of GBM stem cell lines (NCH421k or NCH644, respectively) compared to the sEVs of all other GBM cell lines and astrocytes. VIM mRNA, a target of miR-124-3p and miR-138-5p, was overexpressed in the sEVs of U251 and U87 GBM cell lines compared to the sEVs of GBM stem cell line and also astrocytes. Our results suggest VIM mRNA, miR-9-5p miRNA, and miR-124-3p miRNA could serve as biomarkers of the sEVs of GBM cells.
Language:
English
Keywords:
glioblastoma
,
biomarkers
,
exosomes
,
small extracellular vesicles
,
miRNA
,
miR-9-5p
,
miR-21-5p
,
miR-124-3p
,
miR-138-5p
,
vimentin
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2020
Number of pages:
22 str.
Numbering:
Vol. 21, iss. 22, art. 8491
PID:
20.500.12556/RUL-133849
UDC:
616-006
ISSN on article:
1422-0067
DOI:
10.3390/ijms21228491
COBISS.SI-ID:
36976899
Publication date in RUL:
17.12.2021
Views:
732
Downloads:
151
Metadata:
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Record is a part of a journal
Title:
International journal of molecular sciences
Shortened title:
Int. j. mol. sci.
Publisher:
MDPI
ISSN:
1422-0067
COBISS.SI-ID:
2779162
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
11.11.2020
Secondary language
Language:
Slovenian
Keywords:
glioblastom
,
biomarkerji
,
eksosomi
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0390
Name:
Funkcijska genomika in biotehnologija za zdravje
Funder:
ARRS - Slovenian Research Agency
Project number:
P3-0108
Name:
Diferenciacija urotelijskih celic
Funder:
ARRS - Slovenian Research Agency
Funding programme:
Young Researcher
Funder:
ARRS - Slovenian Research Agency
Project number:
Z3-1869
Name:
Razvoj anti-FREM2 nanotelesa in njegova uporaba pri ciljanju glioblastomskih celic
Funder:
EC - European Commission
Funding programme:
Interreg
Project number:
146
Acronym:
TRANS-GLIOMA
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