20.500.12556/RUL-133849 Analysis of miR-9-5p, miR-124-3p, miR-21-5p, miR-138-5p, and miR-1-3p in glioblastoma cell lines and extracellular vesicles Glioblastoma (GBM), the most common primary brain tumor, is a complex and extremely aggressive disease. Despite recent advances in molecular biology, there is a lack of biomarkers, which would improve GBM's diagnosis, prognosis, and therapy. Here, we analyzed by qPCR the expression levels of a set of miRNAs in GBM and lower-grade glioma human tissue samples and performed a survival analysis in silico. We then determined the expression of same miRNAs and their selected target mRNAs in small extracellular vesicles (sEVs) of GBM cell lines. We showed that the expression of miR-21-5p was significantly increased in GBM tissue compared to lower-grade glioma and reference brain tissue, while miR-124-3p and miR-138-5p were overexpressed in reference brain tissue compared to GBM. We also demonstrated that miR-9-5p and miR-124-3p were overexpressed in the sEVs of GBM stem cell lines (NCH421k or NCH644, respectively) compared to the sEVs of all other GBM cell lines and astrocytes. VIM mRNA, a target of miR-124-3p and miR-138-5p, was overexpressed in the sEVs of U251 and U87 GBM cell lines compared to the sEVs of GBM stem cell line and also astrocytes. Our results suggest VIM mRNA, miR-9-5p miRNA, and miR-124-3p miRNA could serve as biomarkers of the sEVs of GBM cells. glioblastoma biomarkers exosomes small extracellular vesicles miRNA miR-9-5p miR-21-5p miR-124-3p miR-138-5p vimentin glioblastom biomarkerji eksosomi true false true Angleški jezik Slovenski jezik Članek v reviji 2021-12-17 08:14:05 2021-12-17 08:14:09 2022-09-13 03:52:47 0000-00-00 00:00:00 2020 0 0 22 str. iss. 22, art. 8491 Vol. 21 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 1422-0067 10.3390/ijms21228491 36976899 RAZ_Zottel_Alja_2020.pdf RAZ_Zottel_Alja_2020.pdf 1 9F9912E6D7E1416258B4293B1F1B01CE d979b203363d223dbe2dd4e74a3efc4f94c7128b1126b18cee352bf6b8a01530 0f7626c3-5f09-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152201 https://www.mdpi.com/1422-0067/21/22/8491 0 ccd894d2-5f08-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152200 Medicinska fakulteta 0 0 0