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Modulation of Campylobacter jejuni motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by quorum-sensing inhibition
ID Šimunović, Katarina (Author), ID Ramić, Dina (Author), ID Xu, Changyun (Author), ID Smole Možina, Sonja (Author)

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Abstract
Campylobacter jejuni is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of C. jejuni. The aim of this study was to investigate the correlation of QS inhibition with changes in C. jejuni motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the luxS-deficient mutant and (ii) treatment of C. jejuni with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the ∆luxS mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments (n = 15/20) were assayed using Vibrio harveyi BB170 bioluminescence. Moderate positive correlation was shown between C. jejuni QS reduction and reduced motility (τ = 0.492, p = 0.024), adhesion to polystyrene surfaces (τ = 0.419, p = 0.008), and invasion (r = 0.394, p = 0.068). The best overall effect was achieved with a Sedum rosea (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by C. jejuni through modulation of the LuxS (QS) system.

Language:English
Keywords:Campylobacter jejuni, LuxS, quorum-sensing inhibition, AI-2, natural extracts, motility, invasion of INT407 cells, adhesion to polystyrene
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2020
Number of pages:14 str.
Numbering:Vol. 8, iss. 1, art. 104
PID:20.500.12556/RUL-133257 This link opens in a new window
UDC:579.22/.26:547.9:615.33
ISSN on article:2076-2607
DOI:10.3390/microorganisms8010104 This link opens in a new window
COBISS.SI-ID:5147768 This link opens in a new window
Publication date in RUL:18.11.2021
Views:649
Downloads:132
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Record is a part of a journal

Title:Microorganisms
Shortened title:Microorganisms
Publisher:MDPI AG
ISSN:2076-2607
COBISS.SI-ID:523277081 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:11.01.2020

Secondary language

Language:Slovenian
Keywords:patogeni mikroorganizmi, Campylobacter jejuni, protimikrobne snovi, rastlinski izvlečki, medcelično signaliziranje, zaznavanje kvoruma, gibljivost, adhezija

Projects

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P4-0116
Name:Mikrobiologija in biotehnologija živil in okolja

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Funding programme:SLO-USA-2018/2019 bilateral projects

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