Modulation of Campylobacter jejuni motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by quorum-sensing inhibition

Šimunović, Katarina (author), Ramić, Dina (author), Xu, Changyun (author), Smole Možina, Sonja (author)

Campylobacter jejuni is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of C. jejuni. The aim of this study was to investigate the correlation of QS inhibition with changes in C. jejuni motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the luxS-deficient mutant and (ii) treatment of C. jejuni with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the ∆luxS mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments (n = 15/20) were assayed using Vibrio harveyi BB170 bioluminescence. Moderate positive correlation was shown between C. jejuni QS reduction and reduced motility (τ = 0.492, p = 0.024), adhesion to polystyrene surfaces (τ = 0.419, p = 0.008), and invasion (r = 0.394, p = 0.068). The best overall effect was achieved with a Sedum rosea (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by C. jejuni through modulation of the LuxS (QS) system.

• Language: English
• Keywords: Campylobacter jejuni, LuxS, quorum-sensing inhibition, AI-2, natural extracts, motility, invasion of INT407 cells, adhesion to polystyrene
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 14 str.
• Numbering: Vol. 8, iss. 1, art. 104
• PID: 20.500.12556/RUL-133257
• UDC: 579.22/.26:547.9:615.33
• ISSN on article: 2076-2607
• DOI: 10.3390/microorganisms8010104
• COBISS.SI-ID: 5147768
• Publication date in RUL: 18.11.2021

Record is a part of a journal

• Title: Microorganisms
• Shortened title: Microorganisms
• Publisher: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 11.01.2020

Secondary language

• Language: Slovenian
• Keywords: patogeni mikroorganizmi, Campylobacter jejuni, protimikrobne snovi, rastlinski izvlečki, medcelično signaliziranje, zaznavanje kvoruma, gibljivost, adhezija

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0116
• Name: Mikrobiologija in biotehnologija živil in okolja

• Funder: ARRS - Slovenian Research Agency
• Funding programme: SLO-USA-2018/2019 bilateral projects