20.500.12556/RUL-133257 Modulation of Campylobacter jejuni motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by quorum-sensing inhibition Campylobacter jejuni is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of C. jejuni. The aim of this study was to investigate the correlation of QS inhibition with changes in C. jejuni motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the luxS-deficient mutant and (ii) treatment of C. jejuni with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the ∆luxS mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments (n = 15/20) were assayed using Vibrio harveyi BB170 bioluminescence. Moderate positive correlation was shown between C. jejuni QS reduction and reduced motility (τ = 0.492, p = 0.024), adhesion to polystyrene surfaces (τ = 0.419, p = 0.008), and invasion (r = 0.394, p = 0.068). The best overall effect was achieved with a Sedum rosea (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by C. jejuni through modulation of the LuxS (QS) system. Campylobacter jejuni LuxS quorum-sensing inhibition AI-2 natural extracts motility invasion of INT407 cells adhesion to polystyrene patogeni mikroorganizmi Campylobacter jejuni protimikrobne snovi rastlinski izvlečki medcelično signaliziranje zaznavanje kvoruma gibljivost adhezija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-18 14:28:39 2021-11-18 14:28:40 2022-09-12 04:15:31 0000-00-00 00:00:00 2020 0 0 14 str. iss. 1, art. 104 Vol. 8 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:547.9:615.33 2076-2607 10.3390/microorganisms8010104 5147768 RAZ_Simunovic_Katarina_2020.pdf RAZ_Simunovic_Katarina_2020.pdf 1 015A92E012B4B1BDB80C4EF5AEE4A6DF 7c5977763863d154f8d5250d16acda5d752cbba2d4d71eda61164d40641fbb87 98a4ef1d-4873-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151159 https://www.mdpi.com/2076-2607/8/1/104 0 91f4f4d9-4873-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151158 Biotehniška fakulteta 0 0 0