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Evaluation of disease severity and global transcriptome response induced by Citrus bark cracking viroid, Hop latent viroid, and their co-infection in hop (Humulus lupulus L.)
ID
Štajner, Nataša
(
Avtor
),
ID
Radišek, Sebastjan
(
Avtor
),
ID
Kumar Mishra, Ajay
(
Avtor
),
ID
Nath, Vishnu Sukumari
(
Avtor
),
ID
Matoušek, Jaroslav
(
Avtor
),
ID
Jakše, Jernej
(
Avtor
)
PDF - Predstavitvena datoteka,
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(3,59 MB)
MD5: EC4D3892AA331AC615C11AA2FEE771A6
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/1422-0067/20/13/3154
Galerija slik
Izvleček
Viroids are small non-capsidated, single-stranded, covalently-closed circular noncoding RNA replicons of 239–401 nucleotides that exploit host factors for their replication, and some cause disease in several economically important crop plants, while others appear to be benign. The proposed mechanisms of viroid pathogenesis include direct interaction of the genomic viroid RNA with host factors and post-transcriptional or transcriptional gene silencing via viroid-derived small RNAs (vd-sRNAs) generated by the host defensive machinery. Humulus lupulus (hop) plants are hosts to several viroids among which Hop latent viroid (HLVd) and Citrus bark cracking viroid (CBCVd) are attractive model systems for the study of viroid-host interactions due to the symptomless infection of the former and severe symptoms induced by the latter in this indicator host. To better understand their interactions with hop plant, a comparative transcriptomic analysis based on RNA sequencing (RNA-seq) was performed to reveal the transcriptional alterations induced as a result of single HLVd and CBCVd infection in hop. Additionally, the effect of HLVd on the aggressiveness of CBCVd that underlies severe stunting in hop in a mixed infection was studied by transcriptomic analysis. Our analysis revealed that CBCVd infection resulted in dynamic changes in the activity of genes as compared to single HLVd infection and their mixed infection. The differentially expressed genes that are involved in defense, phytohormone signaling, photosynthesis and chloroplasts, RNA regulation, processing and binding; protein metabolism and modification; and other mechanisms were more modulated in the CBCVd infection of hop. Nevertheless, Gene Ontology (GO) classification and pathway enrichment analysis showed that the expression of genes involved in the proteolysis mechanism is more active in a mixed infection as compared to a single one, suggesting co-infecting viroids may result in interference with host factors more prominently. Collectively, our results provide a deep transcriptome of hop and insight into complex single HLVd, CBCVd, and their coinfection in hop-plant interactions.
Jezik:
Angleški jezik
Ključne besede:
Citrus bark cracking viroid
,
co-infection
,
differentially expressed genes
,
Humulus lupulus
,
Hop latent viroid
,
transcriptome profiling
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2019
Št. strani:
21 str.
Številčenje:
Vol. 20, iss. 13, art. 3154
PID:
20.500.12556/RUL-132222
UDK:
577.2
ISSN pri članku:
1422-0067
DOI:
10.3390/ijms20133154
COBISS.SI-ID:
9268089
Datum objave v RUL:
18.10.2021
Število ogledov:
764
Število prenosov:
136
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
International journal of molecular sciences
Skrajšan naslov:
Int. j. mol. sci.
Založnik:
MDPI
ISSN:
1422-0067
COBISS.SI-ID:
2779162
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
01.07.2019
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
hmelj
,
genetika
,
viroidi
,
huda viroidna zakrnelost hmelja
,
izraženost genov
,
transkriptomska analiza
Projekti
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Operational Programme Research, Development and Education
Številka projekta:
CZ.02.2.69/0.0/0.0/16_027/0008357
Akronim:
MEMOBIC
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Czech Science foundation
Številka projekta:
18-10515J/DFG STE 465/10-1
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
L4-6809
Naslov:
Analiza odziva rastlin ob hkratnih okužbah viroidov in identifikacija odpornosti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P4-0077
Naslov:
Kmetijske rastline - genetika in sodobne tehnologije
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
IPMB
Številka projekta:
RVO:60077344
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