Effects of vitamin E and coenzyme Q$_{10}$ supplementation on oxidative stress parameters in untrained leisure horses subjected to acute moderate exercise

Nemec Svete, Alenka (author), Vovk, Tomaž (author), Bohar Topolovec, Mojca (author), Kruljc, Peter (author)

The effects of antioxidant supplements on exercise-induced oxidative stress have not been investigated in untrained leisure horses. We investigated the effects of 14-day supplementation with vitamin E (1.8 IU/kg/day), coenzyme Q$_{10}$ (CoQ$_{10}$; ubiquinone; 800 mg/day), and a combination of both (the same doses as in mono-supplementation) on the blood levels of CoQ$_{10}$, vitamin E, and oxidative stress parameters in untrained leisure horses subjected to acute moderate exercise. Correlations between lipid peroxidation and muscle enzyme leakage were also determined. Forty client-owned horses were included in the study, with 10 horses in each of the antioxidant and placebo (paraffin oil) groups. Blood parameters were measured before supplementation, before and immediately after exercise, and after 24 h of rest. The differences in individual parameters between blood collection times and groups were analysed with linear mixed models (p < 0.05). None of the supplemented antioxidants affected vitamin E and CoQ$_{10}$ concentrations, oxidative stress parameters, or serum muscle enzymes. Lipid peroxidation occurred in horses supplemented with placebo and CoQ$_{10}$ but not in horses supplemented with vitamin E or the combination of both antioxidants. These results suggest that vitamin E alone or in combination with CoQ$_{10}$ prevented lipid peroxidation in untrained leisure horses subjected to acute moderate exercise.

• Language: English
• Keywords: warm-blooded horses, acute moderate exercise, exercise-induced oxidative stress, coenzyme Q$_{10}$, vitamin e, malondialdehyde, creatine kinase, aspartate aminotransferase, antioxidant enzymes, oxidative stress, sports medicine, antioxidants, blood chemical analysis, physical conditioning, animal, horses
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty; FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 17 str.
• Numbering: Vol. 10, iss. 6, art. 908
• PID: 20.500.12556/RUL-132096
• UDC: 636.3.09:612.766.1:577
• ISSN on article: 2076-3921
• DOI: 10.3390/antiox10060908
• COBISS.SI-ID: 65803011
• Publication date in RUL: 12.10.2021

Record is a part of a journal

• Title: Antioxidants
• Shortened title: Antioxidants
• Publisher: MDPI
• ISSN: 2076-3921
• COBISS.SI-ID: 522976025

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 03.06.2021

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0053
• Name: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0189
• Name: Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih