20.500.12556/RUL-132096 Effects of vitamin E and coenzyme Q$_{10}$ supplementation on oxidative stress parameters in untrained leisure horses subjected to acute moderate exercise The effects of antioxidant supplements on exercise-induced oxidative stress have not been investigated in untrained leisure horses. We investigated the effects of 14-day supplementation with vitamin E (1.8 IU/kg/day), coenzyme Q$_{10}$ (CoQ$_{10}$; ubiquinone; 800 mg/day), and a combination of both (the same doses as in mono-supplementation) on the blood levels of CoQ$_{10}$, vitamin E, and oxidative stress parameters in untrained leisure horses subjected to acute moderate exercise. Correlations between lipid peroxidation and muscle enzyme leakage were also determined. Forty client-owned horses were included in the study, with 10 horses in each of the antioxidant and placebo (paraffin oil) groups. Blood parameters were measured before supplementation, before and immediately after exercise, and after 24 h of rest. The differences in individual parameters between blood collection times and groups were analysed with linear mixed models (p < 0.05). None of the supplemented antioxidants affected vitamin E and CoQ$_{10}$ concentrations, oxidative stress parameters, or serum muscle enzymes. Lipid peroxidation occurred in horses supplemented with placebo and CoQ$_{10}$ but not in horses supplemented with vitamin E or the combination of both antioxidants. These results suggest that vitamin E alone or in combination with CoQ$_{10}$ prevented lipid peroxidation in untrained leisure horses subjected to acute moderate exercise. warm-blooded horses acute moderate exercise exercise-induced oxidative stress coenzyme Q$_{10}$ vitamin e malondialdehyde creatine kinase aspartate aminotransferase antioxidant enzymes oxidative stress sports medicine antioxidants blood chemical analysis physical conditioning animal horses true false true Angleški jezik Ni določen Članek v reviji 2021-10-12 13:31:47 2021-10-12 13:31:49 2022-09-12 04:11:02 0000-00-00 00:00:00 2021 0 0 17 str. iss. 6, art. 908 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 636.3.09:612.766.1:577 2076-3921 10.3390/antiox10060908 65803011 RAZ_Nemec_Svete_Alenka_2021.pdf RAZ_Nemec_Svete_Alenka_2021.pdf 1 114F8C0C55D4E18D531897595B3F9907 2e96ea35933f334bdb365bc0a2a56b96c55b466603efb1735658b5b4f3f83971 d22fcde2-ac21-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154912 https://www.mdpi.com/2076-3921/10/6/908 1 fe29209e-2b4f-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149619 Veterinarska fakulteta Fakulteta za farmacijo 0 0 0