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Analysis of the global population structure of Paenibacillus larvae and outbreak investigation of American foulbrood using a stable wgMLST scheme
ID Papić, Bojan (Author), ID Diricks, Margo (Author), ID Kušar, Darja (Author)

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Abstract
Paenibacillus larvae causes the American foulbrood (AFB), a highly contagious and devastating disease of honeybees. Whole-genome sequencing (WGS) has been increasingly used in bacterial pathogen typing, but rarely applied to study the epidemiology of P. larvae. To this end, we used 125 P. larvae genomes representative of a species-wide diversity to construct a stable whole-genome multilocus sequence typing (wgMLST) scheme consisting of 5745 loci. A total of 51 P. larvae isolates originating from AFB outbreaks in Slovenia were used to assess the epidemiological applicability of the developed wgMLST scheme. In addition, wgMLST was compared with the core-genome MLST (cgMLST) and whole-genome single nucleotide polymorphism (wgSNP) analyses. All three approaches successfully identified clusters of outbreak-associated strains, which were clearly separated from the epidemiologically unlinked isolates. High levels of backward comparability of WGS-based analyses with conventional typing methods (ERIC-PCR and MLST) were revealed; however, both conventional methods lacked sufficient discriminatory power to separate the outbreak clusters. The developed wgMLST scheme provides an improved understanding of the intra- and inter-outbreak genetic diversity of P. larvae and represents an important progress in unraveling the genomic epidemiology of this important honeybee pathogen.

Language:English
Keywords:Paenibacillus larvae, American foulbrood, whole-genome sequencing, whole-genome multilocus sequence typing, core-genome multilocus sequence typing, whole-genome single nucleotide polymorphism, genotype, molecular sequence
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Publication status:Published
Publication version:Version of Record
Year:2021
Number of pages:15 str.
Numbering:Vol. 8, art. 582677
PID:20.500.12556/RUL-132077 This link opens in a new window
UDC:577
ISSN on article:2297-1769
DOI:10.3389/fvets.2021.582677 This link opens in a new window
COBISS.SI-ID:53310723 This link opens in a new window
Publication date in RUL:11.10.2021
Views:1167
Downloads:145
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Record is a part of a journal

Title:Frontiers in veterinary science
Shortened title:Front. vet. sci.
Publisher:Frontiers Media
ISSN:2297-1769
COBISS.SI-ID:3969402 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Projects

Funder:ARRS - Slovenian Research Agency
Project number:V4-1804
Name:Ugotavljanje poti širjenja hude gnilobe čebelje zalege z genetsko tipizacijo sevov povzročitelja bolezni

Funder:Other - Other funder or multiple funders
Funding programme:Republic of Slovenia, Ministry of Agriculture, Forestry and Food

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