20.500.12556/RUL-132077
Analysis of the global population structure of Paenibacillus larvae and outbreak investigation of American foulbrood using a stable wgMLST scheme
Paenibacillus larvae causes the American foulbrood (AFB), a highly contagious and devastating disease of honeybees. Whole-genome sequencing (WGS) has been increasingly used in bacterial pathogen typing, but rarely applied to study the epidemiology of P. larvae. To this end, we used 125 P. larvae genomes representative of a species-wide diversity to construct a stable whole-genome multilocus sequence typing (wgMLST) scheme consisting of 5745 loci. A total of 51 P. larvae isolates originating from AFB outbreaks in Slovenia were used to assess the epidemiological applicability of the developed wgMLST scheme. In addition, wgMLST was compared with the core-genome MLST (cgMLST) and whole-genome single nucleotide polymorphism (wgSNP) analyses. All three approaches successfully identified clusters of outbreak-associated strains, which were clearly separated from the epidemiologically unlinked isolates. High levels of backward comparability of WGS-based analyses with conventional typing methods (ERIC-PCR and MLST) were revealed; however, both conventional methods lacked sufficient discriminatory power to separate the outbreak clusters. The developed wgMLST scheme provides an improved understanding of the intra- and inter-outbreak genetic diversity of P. larvae and represents an important progress in unraveling the genomic epidemiology of this important honeybee pathogen.
Paenibacillus larvae
American foulbrood
whole-genome sequencing
whole-genome multilocus sequence typing
core-genome multilocus sequence typing
whole-genome single nucleotide polymorphism
genotype
molecular sequence
true
false
true
Angleški jezik
Ni določen
Članek v reviji
2021-10-11 17:09:11
2021-10-11 17:09:12
2023-03-24 03:37:00
0000-00-00 00:00:00
2021
0
0
15 str.
art. 582677
Vol. 8
2021
0000-00-00
Zaloznikova
Objavljeno
NiDoloceno
0000-00-00
0000-00-00
0000-00-00
577
2297-1769
10.3389/fvets.2021.582677
53310723
RAZ_Papic_Bojan_2021.pdf
RAZ_Papic_Bojan_2021.pdf
1
8E53578103106988C76209F7DA8D8EFA
efb85fc690c4cd29f9467d3c8909deabf12ce8cdeaa2086d8b4b31bd5d02af83
d3a0e6a8-2aa7-11ec-abdb-00155dcfd717
https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149589
https://www.frontiersin.org/articles/10.3389/fvets.2021.582677/full
1
330b079f-2aa5-11ec-abdb-00155dcfd717
https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149588
Veterinarska fakulteta
0
0
0