Genome-wide transcriptomic analysis reveals insights into the response to Citrus bark cracking viroid (CBCVd) in hop (Humulus lupulus L.)

Kumar Mishra, Ajay (author), Kumar, Atul (author), Mishra, Deepti (author), Nath, Vishnu Sukumari (author), Jakše, Jernej (author), Kocábek, Tomáš (author), Kumar Killi, Uday (author), Morina, Filis (author), Matoušek, Jaroslav (author)

Viroids are smallest known pathogen that consist of non-capsidated, single-stranded non-coding RNA replicons and they exploits host factors for their replication and propagation. The severe stunting disease caused by Citrus bark cracking viroid (CBCVd) is a serious threat, which spreads rapidly within hop gardens. In this study, we employed comprehensive transcriptome analyses to dissect host-viroid interactions and identify gene expression changes that are associated with disease development in hop. Our analysis revealed that CBCVd-infection resulted in the massive modulation of activity of over 2000 genes. Expression of genes associated with plant immune responses (protein kinase and mitogen-activated protein kinase), hypersensitive responses, phytohormone signaling pathways, photosynthesis, pigment metabolism, protein metabolism, sugar metabolism, and modification, and others were altered, which could be attributed to systemic symptom development upon CBCVd-infection in hop. In addition, genes encoding RNA-dependent RNA polymerase, pathogenesis-related protein, chitinase, as well as those related to basal defense responses were up-regulated. The expression levels of several genes identified from RNA sequencing analysis were confirmed by qRT-PCR. Our systematic comprehensive CBCVd-responsive transcriptome analysis provides a better understanding and insights into complex viroid-hop plant interaction. This information will assist further in the development of future measures for the prevention of CBCVd spread in hop fields.

• Language: English
• Keywords: Citrus bark cracking viroid, differentially expressed genes, hop, pathogen, transcriptome analysis, viroids
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2018
• Number of pages: 19 str.
• Numbering: Vol. 10, iss. 10, art. 570
• PID: 20.500.12556/RUL-132062
• UDC: 577.2
• ISSN on article: 1999-4915
• DOI: 10.3390/v10100570
• COBISS.SI-ID: 9078905
• Publication date in RUL: 11.10.2021

Record is a part of a journal

• Title: Viruses
• Shortened title: Viruses
• Publisher: MDPI
• ISSN: 1999-4915
• COBISS.SI-ID: 517597977

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 18.10.2018

Secondary language

• Language: Slovenian
• Keywords: genetske transformacije, viroid razpokanosti skorje agrumov, CBCVd, huda viroidna zakrnelost hmelja, Humulus lupulus, hmelj, transkriptomska analiza, različno izraženi geni