20.500.12556/RUL-132062 Genome-wide transcriptomic analysis reveals insights into the response to Citrus bark cracking viroid (CBCVd) in hop (Humulus lupulus L.) Viroids are smallest known pathogen that consist of non-capsidated, single-stranded non-coding RNA replicons and they exploits host factors for their replication and propagation. The severe stunting disease caused by Citrus bark cracking viroid (CBCVd) is a serious threat, which spreads rapidly within hop gardens. In this study, we employed comprehensive transcriptome analyses to dissect host-viroid interactions and identify gene expression changes that are associated with disease development in hop. Our analysis revealed that CBCVd-infection resulted in the massive modulation of activity of over 2000 genes. Expression of genes associated with plant immune responses (protein kinase and mitogen-activated protein kinase), hypersensitive responses, phytohormone signaling pathways, photosynthesis, pigment metabolism, protein metabolism, sugar metabolism, and modification, and others were altered, which could be attributed to systemic symptom development upon CBCVd-infection in hop. In addition, genes encoding RNA-dependent RNA polymerase, pathogenesis-related protein, chitinase, as well as those related to basal defense responses were up-regulated. The expression levels of several genes identified from RNA sequencing analysis were confirmed by qRT-PCR. Our systematic comprehensive CBCVd-responsive transcriptome analysis provides a better understanding and insights into complex viroid-hop plant interaction. This information will assist further in the development of future measures for the prevention of CBCVd spread in hop fields. Citrus bark cracking viroid differentially expressed genes hop pathogen transcriptome analysis viroids genetske transformacije viroid razpokanosti skorje agrumov CBCVd huda viroidna zakrnelost hmelja Humulus lupulus hmelj transkriptomska analiza različno izraženi geni true false true Angleški jezik Slovenski jezik Članek v reviji 2021-10-11 08:23:51 2021-10-11 08:23:52 2022-09-12 04:10:51 0000-00-00 00:00:00 2018 0 0 19 str. iss. 10, art. 570 Vol. 10 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 1999-4915 10.3390/v10100570 9078905 RAZ_Kumar_Mishra_Ajay_2018.pdf RAZ_Kumar_Mishra_Ajay_2018.pdf 1 12DE70BE3D088E779C1413898C0CC3FD 4400caff13e1c49e3c5e541b8eaaf075bc6a6c319e4608b8b6cd9458b726c1e9 bc8a0155-2a5b-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149562 https://www.mdpi.com/1999-4915/10/10/570 0 b5198a0b-2a5b-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149561 Biotehniška fakulteta 0 0 0